Rabbit anti sting pab

PEDV-permissive Vero cells and human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific) and 100 U/mL of penicillin plus 100 g/mL streptomycin. IPEC-DQ cells were cultured in Roswell Park Memorial Institute (RPMI) medium (Hyclone Laboratories, Logan, UT, USA) containing 10% FBS with penicillin/streptomycin. All cells were maintained at 37 °C with 5% CO₂ in a humidified incubator. The PEDV strain used in this study was a mutant strain of PEDV AJ1102 (GenBank: JX188454.1). Poly(I:C)-LMW, poly(I:C)-HMW, 2′3′-cGAMP and poly(dA:dT) were all obtained from InvivoGen (Hongkong, China). Mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mAb, mouse anti-green fluorescence protein (GFP) mAb, and mouse anti-β-Actin mAb were all brought from TransGen (Beijing, China). Mouse anti-MAVS mAb was from Santa Cruz Biotechnology (Dallas, TX, USA), rabbit anti-TRIF pAb was from Biodargon (Beijing, China), and rabbit anti-STING pAb was bought from Proteintech (Wuhan, China).

Mouse antiactin monoclonal antibody (mAb), mouse anti-GFP mAb, mouse anti-HA mAb, and mouse anti-His mAb were all from Transgen Biotech. The mouse anti-GST mAb was bought from Abmart Biotech. The rabbit anti-TBK1 (D1B4), anti–phospho-TBK1 (D52C2), anti-IRF3 (D614C), anti-IκBα (44D4), and anti-HA (C29F4) mAbs were bought from Cell Signaling Technology. The rabbit anti–phospho-IRF3 mAb and anti-IKKε mAb were acquired from ThermoFisher Scientific and The HUABIO, respectively. Rabbit anti-STING pAb, mouse anti-mCherry, and anti-Myc mAbs were purchased from ProteinTech. The rabbit anti-ISG56-pAb was homemade in our laboratory. The horseradish peroxidase antimouse immunoglobulin G and horseradish peroxidase anti-rabbit immunoglobulin G were purchased from Sangon Biotech.