Anti wnt8a

Manufactured by R&D Systems

Anti-Wnt8a is a recombinant antibody that binds and neutralizes the Wnt8a protein. Wnt8a is a member of the Wnt family of secreted glycoproteins that play a role in various cellular processes.

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Lab products found in correlation

Luminol reagent, by Santa Cruz Biotechnology (2 mentions) Anti gapdh antibody, by GeneTex (2 mentions) Anti gsk3β, by Cell Signaling Technology (2 mentions) Anti β catenin, by Cell Signaling Technology (2 mentions) Dc protein assay, by Bio-Rad (2 mentions) Anti wnt3a, by R&D Systems (2 mentions)

Spelling variants of this product

WNT8A (2 citations)

2 protocols using anti wnt8a

Western Blot Analysis of Wnt Pathway Proteins

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Cells were lysed in RIPA buffer containing protease and phosphatase inhibitors. The cell suspension was centrifuged at 15,000g for 15 min to remove debris, and protein levels in the supernatant quantified with BioRad’s DC protein assay. Proteins (5–10 μg) were electrophoresed and transferred per standard methods, prior to blocking in 5% milk/TBST. All primary antibodies were diluted 1:1000 in TBST and were incubated overnight at 4 °C. The antibodies included anti-β-catenin (Cell Signaling no. 9562), anti-GSK3β (Cell Signaling no. 9315), anti-Wnt3a (R&D Systems no. MAB1324-050), and anti-Wnt8a (R&D Systems no. AF2248). Anti-GAPDH antibody (Genetex no. GT-239) was used as a loading control. The appropriate secondary HRP-conjugated antibody (1:2000 dilution, Santa Cruz) was incubated with the blot and proteins were detected using Luminol reagent (Santa Cruz). Band intensity was determined by densitometry.

Bain L.J., Liu J.T, & League R.E. (2016). Arsenic inhibits stem cell differentiation by altering the interplay between the Wnt3a and Notch signaling pathways. Toxicology Reports, 3, 405-413.

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Western Blot Analysis of Wnt Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in RIPA buffer containing protease and phosphatase inhibitors. The cell suspension was centrifuged at 15,000xg for 15 minutes to remove debris, and protein levels in the supernatant quantified with BioRad's DC protein assay. Proteins (5-10μg) were electrophoresed and transferred per standard methods, prior to blocking in 5% milk/TBST. All primary antibodies were diluted 1:1000 in TBST and were incubated overnight at 4°C. The antibodies included anti-β-catenin (Cell Signaling no. 9562), anti-GSK3β (Cell Signaling no. 9315), anti-Wnt3a (R&D Systems no. MAB1324-050), and anti-Wnt8a (R&D Systems no. AF2248). Anti-GAPDH antibody (Genetex no. GT-239) was used as a loading control. The appropriate secondary HRP-conjugated antibody (1:2000 dilution, Santa Cruz) was incubated with the blot and proteins were detected using Luminol reagent (Santa Cruz). Band intensity was determined by densitometry.

Bain L.J., Liu J.T, & League R.E. (2016). Arsenic inhibits stem cell differentiation by altering the interplay between the Wnt3a and Notch signaling pathways. Toxicology Reports, 3, 405-413.

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