Cdna synthesis supermix

Manufactured by Quanta Biosciences

The cDNA Synthesis SuperMix is a ready-to-use solution for the reverse transcription of RNA into complementary DNA (cDNA). It contains all the necessary components for efficient cDNA synthesis, including a reverse transcriptase enzyme, reaction buffer, and dNTPs.

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Lab products found in correlation

Prism 7500 sequence detection system, by Thermo Fisher Scientific (2 mentions) Sybr green pcr master mix, by Thermo Fisher Scientific (2 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

QScript cDNA XLT cDNA Synthesis SuperMix QScript™ cDNA SuperMix synthesis kit QScript cDNA Synthesis Supermix

2 protocols using cdna synthesis supermix

Quantitative Gene Expression Analysis

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At the end of the experiments, total RNA was isolated from explanted fibroblasts, skin biopsies, or organotypic skin rafts and reverse-transcribed to complementary DNA (cDNA) with SuperMix as described (cDNA Synthesis SuperMix, Quanta BioSciences) (15 (link)). The products (50 ng) were amplified with SYBR Green PCR Master Mix (Applied Biosystems) on an Applied Biosystems 7500 Prism Sequence Detection System. The sequence of the primers is shown in Table 1. MicroRNA was isolated from confluent fibroblasts with the mirVana miRNA Isolation Kit (Ambion/Applied Biosystems) and amplified with TaqMan probes (Applied Biosystems). Levels of miRNA were determined by qPCR with Applied Biosystems 7500 Prism Sequence Detection System (15 (link)). Data were normalized to GAPDH RNA, and fold change in samples was calculated as 2^−ΔΔCt {2−[(Ct target − Ct GAPDH) treatment − (Ct target − Ct GAPDH) nontreatment]}.

Bhattacharyya S., Tamaki Z., Wang W., Hinchcliff M., Hoover P., Getsios S., White E.S, & Varga J. (2014). FibronectinEDA Promotes Chronic Cutaneous Fibrosis Through Toll-like Receptor Signaling. Science translational medicine, 6(232), 232ra50.

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Quantitative PCR Analysis of Fibroblast Gene Expression

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At the end of experiments, total RNA from SSc, healthy adult, and foreskin fibroblasts was isolated and reverse-transcribed to cDNA (cDNA Synthesis Supermix; Quanta Biosciences). Products (100 ng) were amplified using SYBR Green PCR Master Mix (Applied Biosystems) on an Applied Biosystems 7500 Prism Sequence Detection System. Data were normalized to internal control GAPDH RNA and are represented as the fold change (37 (link)). Primers used are shown in Table 3.

Bale S., Verma P., Yalavarthi B., Scarneo S.A., Hughes P., Amin M.A., Tsou P.S., Khanna D., Haystead T.A., Bhattacharyya S, & Varga J. (2023). Pharmacological inhibition of TAK1 prevents and induces regression of experimental organ fibrosis. JCI Insight, 8(14), e165358.

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