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P jnk antibody

Manufactured by Santa Cruz Biotechnology
Sourced in United States

The P-JNK Antibody is a reagent used in research applications to detect the phosphorylated form of c-Jun N-terminal kinase (JNK). JNK is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in various cellular processes, including stress response and apoptosis. The P-JNK Antibody can be used to study the activation and signaling of the JNK pathway in biological samples.

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2 protocols using p jnk antibody

1

PC12 Cell Culture and Signaling Pathway Analysis

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PC12 cells were obtained from Pasteur Institute (Tehran, Iran), DMEM (Gibco, Grand Island, NY,USA), p-Akt1/2/3 Antibody (Ser 473): sc-7985 (SANTA CRUZ, CA, USA), p-p38 Antibody (Tyr 182): sc-101759 (SANTA CRUZ, CA, USA), p38α Antibody (N-20): sc-728 (SANTA CRUZ, CA, USA), p-JNK Antibody (14.Thr 183/Tyr 185): sc-293136 (SANTA CRUZ, CA, USA), JNK1/3 Antibody (C-17): sc-474, RIPA Buffer (Sigma-Aldrich, New York, NY, USA), Anti-Protease Cocktail (Sigma-Aldrich, New York, NY, USA), Acrylamide and bisAcrylamide (Sigma-Aldrich, New York, NY, USA).
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2

Protein Expression Analysis via Western Blot

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Total proteins were extracted using a protein extraction kit (Invitrogen, Carlsbad, USA), and Western blotting was performed according to the manufacturer’s instructions. Antibody concentrations were applied as follows: p-GSK-3β antibody (1:1000), p-JNK antibody (1:1000), p-NF-κB p65 antibody (1:1000), IκBα antibody (1:1000) and β-actin (1:1000, Santa Cruz, USA). The results are expressed relative to the control (control, set as 1.0).
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