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Au640 automatic biochemical analyzer

Manufactured by Olympus
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Sourced in Japan

The AU640 is an automatic biochemical analyzer designed for clinical laboratory applications. It is capable of performing a variety of biochemical tests on patient samples. The AU640 provides automated analysis of various parameters to support clinical diagnostics.

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7 protocols using au640 automatic biochemical analyzer

1

Biochemical Analysis of Rat Serum

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After TFN treatment for four weeks, rats in all groups were anesthetized with 10% chloral hydrate (4.0 ml/kg) on the 29th day. Blood samples were collected from the aorta abdominalis and transferred into the tube with heparin sodium. Blood samples were subsequently centrifuged at 3,500 rpm for 15 min at 4°C and the upper serum was collected for IL-1β, TNF-α, SOD, MDA, Scr, and BUN analysis. Olympus AU640 automatic biochemical analyzer was applied to disclose Scr and BUN levels. The blood of IL-1β, TNF-α, SOD, and MDA content was detected by ELISA according to the instruction in the kits.
Ren L., Guo H.N., Yang J., Guo X.Y., Wei Y.S, & Yang Z. (2021). Dissecting Efficacy and Metabolic Characteristic Mechanism of Taxifolin on Renal Fibrosis by Multivariate Approach and Ultra-Performance Liquid Chromatography Coupled With Mass Spectrometry-Based Metabolomics Strategy. Frontiers in Pharmacology, 11, 608511.
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2

Uric Acid Quantification via Uricase

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The uricase method was used to detect uric acid levels. We got the proband's blood under fasting. The blood was centrifuged at 3000 r/min for 10 min. Then the supernatant was taken and detected by an Olympus AU640 automatic biochemical analyzer. The kit was purchased from Mindray (Shenzhen, China).
Du P., Hassan R.N., Luo H., Xie J., Zhu Y., Hu Q., Yan J, & Jiang W. (2021). Identification of a novel SUOX pathogenic variants as the cause of isolated sulfite oxidase deficiency in a Chinese pedigree. Molecular Genetics & Genomic Medicine, 9(2), e1590.
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3

High-Fat Diet Induces Obesity

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After adaptive feeding for one week, the C57bl/6j mice were fed with high fat forage (fat content about 36%) for 8 weeks up to the obesity model standard. The mice weight was gained at the 4th and 8th week, respectively. The bloods in each group were collected at the 4th and 8th weekend to measure the serum uric acid levels by Olympus AU 640 automatic biochemical analyzer.
Gao Y., Sun J., Zhang Y., Shao T., Li H., Wang M., Zhang L., Bian H., Wen C., Xie Z, & Lv H. (2020). Effect of a Traditional Chinese Medicine Formula (CoTOL) on Serum Uric Acid and Intestinal Flora in Obese Hyperuricemic Mice Inoculated with Intestinal Bacteria. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 8831937.
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4

Homocysteine Determination in Patients

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The enzymatic cycling assay was used to determine the level of homocysteine in the patient (Roberts & Roberts, 2004). The patient serum was obtained as described in 2.5.2 and detected by an Olympus AU640 automatic biochemical analyzer. The homocysteine detection kit was purchased from Medicalsystem (Zhejiang, China).
Du P., Hassan R.N., Luo H., Xie J., Zhu Y., Hu Q., Yan J, & Jiang W. (2021). Identification of a novel SUOX pathogenic variants as the cause of isolated sulfite oxidase deficiency in a Chinese pedigree. Molecular Genetics & Genomic Medicine, 9(2), e1590.
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5

Thyroid and Metabolic Biomarker Analysis

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The clinical characteristics of the study population include in serum free triiodothyronine (FT3), serum free thyroxine (FT4), alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride content (TG) and total cholesterol (TC). These were measured by Olympus AU640 automatic biochemical analyzer (Olympus Corporation, Tokyo, Japan).
Zheng Y., Zhao C., Zhang N., Kang W., Lu R., Wu H., Geng Y., Zhao Y, & Xu X. (2018). Serum microRNA miR-206 is decreased in hyperthyroidism and mediates thyroid hormone regulation of lipid metabolism in HepG2 human hepatoblastoma cells. Molecular Medicine Reports, 17(4), 5635-5641.
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6

Liver Enzyme and Lipid Analysis

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Liver tissue (200 mg) was homogenized in 2 ml 0.7% saline and centrifuged at 3,000 × g for 15 min at 4°C. The protein-containing supernatant was used to measure enzyme activities (AMPK, ACC, FAS, CPT-1, and ACO) using customized ELISA kits (Zike Biological Technology Co., Ltd., Shenzhen, China) according to the manufacturer’s instructions.
The concentration of plasma triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and total cholesterol (TC) was measured within 24 h of blood collection in a refrigerator by an Olympus AU640 automatic biochemical analyzer (Olympus, Tokyo, Japan).
Hong M., Li N., Li J., Li W., Liang L., Li Q., Wang R., Shi H., Storey K.B, & Ding L. (2019). Adenosine Monophosphate-Activated Protein Kinase Signaling Regulates Lipid Metabolism in Response to Salinity Stress in the Red-Eared Slider Turtle Trachemys scripta elegans. Frontiers in Physiology, 10, 962.
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7

Serum Biochemistry Analysis Protocol

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Fasting venous blood (10 ml) were collected for the detection of serum biochemistry, and other indicators. All biochemical analyses were performed using the same Au640 automatic biochemical analyzer produced by Olympus Company in Japan.
Qiu P., Du J., Zhang C., Li M., Li H, & Chen C. (2023). Increased risk of reflux esophagitis in non-obese individuals with nonalcoholic fatty liver disease: a cross-sectional study. Annals of Medicine, 55(2), 2294933.
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