Regarding rabbit polyclonal primary antibodies, anti-pan-fimbrin was a generous gift from Paul Matsudaira (National University of Singapore),39 (link) and anti-human Tmod1 was prepared in our laboratory.30 (link) With respect to mouse monoclonal primary antibodies, anti-α-actinin (nonsarcomeric, Actn1) was from Sigma-Aldrich Corp. (A5044; St. Louis, MO, USA), anti-Arp3 was from BD Biosciences (612134; San Jose, CA, USA), anti-ezrin from Sigma-Aldrich Corp. (E8897), and anti-β2-spectrin from BD Biosciences (612563). Rat monoclonal primary antibody anti-N-cadherin was a generous gift from Dietmar Vestweber (Max-Planck-Institute for Molecular Biomedicine).40 (link) Secondary antibodies were Alexa-488–conjugated goat anti-rabbit (A11008; Thermo Fisher Scientific, Grand Island, NY, USA), Alexa-488–conjugated goat anti-mouse (115-545-166, minimal cross-reaction; Jackson ImmunoResearch, West Grove, PA, USA), Alexa-647–conjugated goat anti-rat (112-605-167, minimal cross-reaction; Jackson ImmunoResearch), and Alexa-647–conjugated goat anti-mouse IgG (A21236; Thermo Fisher Scientific). Rhodamine-phalloidin (R415, Thermo Fisher Scientific) was used to stain F-actin, and Hoechst 33258 (B2883; Sigma-Aldrich Corp.) stained nuclei.
Anti ezrin
Manufactured by Merck Group
Sourced in United States
Anti-ezrin is a laboratory reagent used in research applications. It is an antibody that specifically binds to the ezrin protein, which is involved in the regulation of cell structure and function. The core function of Anti-ezrin is to enable the detection and analysis of ezrin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Rhodamine phalloidin, by Thermo Fisher Scientific (3 mentions)
Anti α actinin, by Merck Group (1 mentions)
Alexa 488 conjugated goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Alexa488 conjugated goat anti mouse, by Jackson ImmunoResearch (1 mentions)
Alexa 647 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti vimentin, by Merck Group (1 mentions)
Chemidoc xr, by Bio-Rad (1 mentions)
Ecl select western blotting detection reagent, by Bio-Rad (1 mentions)
Quantity one image software 4, by Bio-Rad (1 mentions)
Radioimmunoprecipitation assay (ripa), by Thermo Fisher Scientific (1 mentions)
A32959, by Thermo Fisher Scientific (1 mentions)
Anti cd99, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
3 protocols using anti ezrin
1
Immunofluorescence Antibody Staining Protocol
2
Synaptosome and Gliosome Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aliquots of total crude preparations (the supernatants collected before stratification on the Percoll gradient, containing both synaptosomes and gliosomes), obtained from both control and SMOX mice, were lysed in Laemmli’s sample buffer by sonication and heated for 5 min at 95 °C. Total proteins (25 μg/lane) were then separated by 8% SDS-PAGE, followed by Western blot. Nitrocellulose membrane was blocked by incubation for 1 h at room temperature with 5% skim milk powder in PBS, containing 0.05% Tween-20. Successively, the membrane was incubated for 16 h at 4 °C with one of the following primary antibodies: anti-vimentin (1:500, Sigma-Aldrich, Milano, Italy), anti-ezrin (1:2000, Sigma-Aldrich), or anti-β-actin (1:1000, Santa Cruz Biotech, Dallas, USA). Suitable peroxidase-conjugated secondary antibodies (1 h at 22 °C) were used. Immunoreactive signals were developed using ECL Select™ Western Blotting Detection Reagent, acquired and quantified using ChemiDoc™ XRS equipped with Quantity One Image Software 4.6.1 (Bio-Rad Laboratories Srl, Segrate, Italy). The membrane was stripped and re-probed.
3
Protein Analysis of Cell and EV Lysates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lysis of cells and EVs was performed using the radioimmunoprecipitation assay (RIPA) buffer (89900, Thermo Fisher Scientific) supplemented with protease and phosphatase inhibitors (A32959, Thermo Fisher Scientific). An equal amount of proteins were run on SDS gels under denaturing conditions and blotted onto nitrocellulose membranes. The membranes were incubated overnight at 4 °C with the following primary antibodies: anti-CD99 (sc-53148; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Ezrin (E8897; Sigma-Aldrich); anti-XAGE1A (A61802; Epigentek, Farmingdale, NY); anti-GAPDH (5174; Cell Signaling); anti-GPI (MA515396; Thermo Fisher Scientific), anti-IGFALS (sc-377131; Santa Cruz Biotechnology).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!