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P aminohippuric acid

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P-aminohippuric acid is a chemical compound used as a marker in medical and biological research. It is a colorless, crystalline substance that is soluble in water and organic solvents. P-aminohippuric acid is primarily used as a tracer to measure renal plasma flow and glomerular filtration rate in various medical and physiological studies.

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Lab products found in correlation

Probenecid, by Merck Group (2 mentions) Glycine, by Merck Group (1 mentions) Quinidine, by Merck Group (1 mentions) Cephalexin, by Merck Group (1 mentions) L histidine, by Merck Group (1 mentions) Carnosine, by Merck Group (1 mentions) Dimethyl amiloride, by Merck Group (1 mentions) Inulin 5000, by Merck Group (1 mentions) Hyamine hydroxide, by MP Biomedicals (1 mentions) 14c inulin 5000, by Moravek Biochemicals (1 mentions) Tetraethylammonium, by Merck Group (1 mentions) Cephalothin, by Merck Group (1 mentions) Cytoscint scintillation solution, by MP Biomedicals (1 mentions) Penicillin streptomycin, by Quality Biological (1 mentions) Carnitine, by Merck Group (1 mentions) Trypsin solution, by Quality Biological (1 mentions) Formic acid, by Merck Group (1 mentions) Phosphate buffered saline pbs, by Quality Biological (1 mentions) 3 hydroxybutyrate, by Merck Group (1 mentions) Rpmi 1640 medium, by Quality Biological (1 mentions)

4 protocols using p aminohippuric acid

1

Radiolabeled Cefadroxil Uptake Assay

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[3H]Cefadroxil (0.7 Ci/mmol) and [14C]inulin 5000 (1.1 mCi/g) were purchased from Moravek Biochemicals and Radiochemicals (Brea, CA). Unlabeled cefadroxil, glycylproline (GlyPro), glycyl-glycyL-histidine (GlyGlyHis), glycine, L-histidine, probenecid, p-aminohippuric acid (PAH), tetraethylammonium (TEA), quinidine, N1-methylnicotinamide (NMN), carnosine, cephalexin, cephalothin, dimethylamiloride (DMA) and inulin 5000 were purchased from Sigma-Aldrich (St. Louis, MO). CytoScint™ scintillation solution and hyamine hydroxide were purchased from MP Biomedicals (Solon, OH). All other chemicals were acquired from standard sources.
Hu Y, & Smith D.E. (2016). Species Differences in the Pharmacokinetics of Cefadroxil as Determined in Wildtype and Humanized PepT1 Mice. Biochemical pharmacology, 107, 81-90.
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2

Synthesis and Characterization of (R,R')-MNF Compound

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(R,R’)-MNF was synthesized as previously described.14 (link) Lactate (purity ≥ 98%), 3-hydroxybutyrate (purity ≥ 98%), carnitine (purity ≥ 98%), p-aminohippuric acid (purity ≥ 98%), human recombinant insulin (dry powder), methanol (HPLC grade), acetonitrile (HPLC grade) and formic acid were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), RPMI-1640 medium, Eagle’s Minimum Essential Medium (EMEM), trypsin solution, phosphate-buffered saline (PBS), fetal bovine serum (FBS), 100X solutions of sodium pyruvate (100 mM), L-glutamine (200 mM), and penicillin/streptomycin (a mixture of 10,000 units/mL) were obtained from Quality Biological (Gaithersburg, MD, USA).
Bernier M., Catazaro J., Singh N.S., Wnorowski A., Boguszewska-Czubara A., Jozwiak K., Powers R, & Wainer I.W. (2017). GPR55 receptor antagonist decreases glycolytic activity in PANC-1 pancreatic cancer cell line and tumor xenografts. International journal of cancer, 141(10), 2131-2142.
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3

Fluorescent Probes for Biological Studies

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Sulforhodamine 101 (Sulfo101) was purchased from Chemodex (St. Gallen, Switzerland), 8-(2-[fluoresceinyl]aminoethylthio)adenosine-3′,5′-cyclic monophosphate (8-fluo-cAMP) was purchased from Biolog Life Science (Bremen, Germany), a fluorescent cyclosporine A derivative [N-ϵ(4-nitrobenzofurazan-7-yl)-D-Lys8]cyclosporine A (NBD-CsA) was synthesized as previously described (18 (link)), FA-polyethylene glycol PEG-2kDa-FITC was purchased from Biochempeg (Watertown, MA), and NHS-PEG5-Mal and NHS-PEG40-Mal were obtained from NOF (Tokyo, Japan). SAMSA fluorescein and NHS-TRITC were purchased from Thermo Fisher Scientific (Waltham, MA). Agarose, probenecid, erythromycin, verapamil hydrochloride, p-aminohippuric acid, MK-571 sodium salt hydrate, zosuquidar hydrochloride, saquinavir, indinavir sulfate salt hydrate, folic acid, fluorescein sodium salt (Fluo), FITC-dextran (40, 70, or 150 kDa), 1-phenyl-2-thiourea (PTU), and ethyl-3-aminobenzoate methanesulfonate (MS-222, tricaine) were purchased from Sigma-Aldrich (Buchs, Switzerland).
Bolten J.S., Pratsinis A., Alter C.L., Fricker G, & Huwyler J. (2022). Zebrafish (Danio rerio) larva as an in vivo vertebrate model to study renal function. American Journal of Physiology - Renal Physiology, 322(3), F280-F294.
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4

Bunyamwera Virus Culture and Compound Screening

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Vero cells (CCL-81) were obtained from the American Type Culture Collection and grown in DMEM (Sigma-Aldrich, St. Louis, MO, USA; D6429) supplemented with 10% fetal bovine serum (FBS, Reactiva S.A., Barcelona, Spain), 1% MEM nonessential amino acids (Sigma-Aldrich; M7145), 1% L-glutamine (Merck, Rahway, NJ, USA; 59202C) and 1% penicillin/streptomycin (Sigma-Aldrich; P4333).
Bunyamwera virus (BUNV, VR-87) was obtained from the American Type Culture Collection (Virginia, WV, USA). Propagation and production of BUNV stocks was performed in Vero cells as previously described [9 (link),37 (link)]. Virus titers measured as plaque forming units, or PFU per milliliter, were determined by standard plaque assay in Vero cells using an agar overlay [37 (link)].
acetylsalicylic acid (A5376), myricetin (M6760), L-phenylalanine (5482), p-aminohippuric acid (A1422) and silibinin A (S0417) were purchased from Sigma-Aldrich, dissolved in ethanol (acetylsalicylic acid and myricetin), water (L-phenylalanine and p-aminohippuric acid) or dimethyl sulfoxide (DMSO) (silibinin A), as recommended by the manufacturer, to prepare stock solutions and stored at −20 °C.
Fernández-Sánchez S.Y., Cerón-Carrasco J.P., Risco C, & Fernández de Castro I. (2023). Antiviral Activity of Acetylsalicylic Acid against Bunyamwera Virus in Cell Culture. Viruses, 15(4), 948.
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