Supelco sp 2380

The fatty acids composition was determined by the conversion of oil to fatty acid methyl esters (FAMEs) according to the modified method described by Zahran and Tawfeuk (2019) . FAMEs were separated with an HP 6890 plus gas chromatography (Hewlett Packard, USA), using a capillary column Supelco™ SP-2380 (60 m×0.25 mm×0.20 μm), (Sigma-Aldrich, USA), Detector (FID) and the injector and detector temperatures were 250°C. The column temperature was 140°C (hold for 5 min) and raised to 240°C, at rate of 4°C/min, and holds at 240°C for 10 min. The carrier gas was helium at flow rate 1.2 mL min -1 . FAMEs were identified by comparing their relative and absolute retention times to those authentic standards of FAMEs (SupelcoTM 37component FAME mix). The fatty acid composition was reported as a relative percentage of the total peak area.

The fatty acid composition was determined by the conversion of oil to Fatty Acid Methyl Esters (FAMEs) according to the modified method of Zahran and Tawfeuk [19] . FAMEs were analyzed using an HP 6890 plus Gas chromatography (GC) (Hewlett Packard, USA), a capillary column Supelco™ SP-2380 (60 m×0.25 mm×0.20 μm), (Sigma-Aldrich, USA), Detector (FID) while the injector and detector temperature were 250°C. The column temperature was 140°C (hold for 5 min) and rise to 240°C at 4°C/min., and hold at 240°C for 10 min. The carrier gas was helium at a flow rate of 1.2 mL min -1 . FAMEs were identified by comparing their relative and absolute retention times to those authentic standards of FAMEs (Supelco TM 37component FAME mix). The fatty acid composition was reported as a relative percentage of the total peak area.