Anti rabbit ig hrp

Antibodies to p-ERK1/2, ERK1/2, p-T308-AKT, AKT, p-Y705-STAT3, JAK1 were purchased from Cell Signaling Technology (Beverly, MA, USA). STAT3 and p-T1022-JAK1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies to HAS2 and C5R1 were purchased from Abcam (Cambridge, UK). 4,6-diamidino-2-phenylindole (DAPI) were purchased form Sigma (St Louis, MO, USA). rh-C5a protein and antibodies to CD105 and C5a were purchased from R&D Systems (Minneapolis, MN, USA). Chemical inhibitors specific to MEK (U0126), PI3K (LY294002), JAK1 (P6) and STAT3 (WP1066) were purchased from Calbiochem (San Diego, CA, USA). Anti-Goat Alexa Fluor 488, anti-mouse Alexa Fluor 546 were purchased from Invitrogen (Carlsbad, CA, USA). Anti-mouse IgG-HRP, anti-goat IgG-HRP and anti-rabbit Ig-HRP were purchased from Santa Cruz biotechnology (Santa Cruz, CA, USA). HA (Low molecular weight; 15-40 kDa) was purchased from R&D systems (Minneapolis, MN, USA).

Antibodies to pan-RAS (ab52939), H-RAS (ab97488), N-RAS (ab77392), K-RAS (ab137739), Raf1 (ab137435), p-JAK1 (sc-101716), p-ERK1/2 (sc-7383), AKT (sc-5298), STAT3 (sc-482), JAK1 (sc-7228), SRC (sc-8056), OCT4 (sc-9081), SOX2 (ab97959) and β-actin (sc-47778), anti-mouse IgG-HRP, anti-goat IgG-HRP, and anti-rabbit Ig-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies to Anti-Goat Alexa Fluor 488, and anti-mouse Alexa Fluor 546 were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies to ERK (4695), CD44 (ab157107), Nanog (ab21624), Slug (ab27568), Zeb1 (ab124512), Vimentin (ab8978), and Fibronectin (ab6329) were purchased from Abcam (Cambridge, UK). Antibodies MEK1/2 (8727), p-MEK1/2 (9121), p-JNK (9251), JNK (9252), p-Src (9211S), p-AKT (4060), Snail (3879S), P38 (9212), p-P38 (9211S) and p-STAT3 (9131) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies to GPR110 were purchased from LSbio. Antibodies to Active-RAS were purchased from NewEast, Antibodies to p-Raf1 were purchased from MyBioSource. Antibodies to Ki-67 were purchased from Actis. Antibodies to N-cadherin and β-catenin were purchased from BD biosciences. Vector of GPR110 were purchased from origene (ADGRF1, NM_153840, RC222706).