Immunohistochemistry was performed according to standard protocols, and the following primary antibodies were used: pERK (rabbit, 1: 200, Cell Signaling, Danvers, MA, USA), Alexa Fluor® 488 (donkey anti-rabbit IgG, 1:1000, Molecular Probes, Waltham, MA), CGRP antibody (goat, 1:300, Abcam, Cambridge, MA), CCR2 antibody (rabbit, 1:300, Novus Biologicals, Centennial, CO, USA), Alexa Fluor® 594 (donkey anti-rabbit IgG, 1:1000, Abcam), pERK antibody (mouse, 1: 300, Abcam), and Alexa Fluor® 594 (donkey anti-mouse IgG, 1:1000, Abcam).
Perk antibody
Manufactured by Abcam
Sourced in United States, United Kingdom
The PERK antibody is a tool for the detection and analysis of PERK (Protein Kinase R (PKR)-Like Endoplasmic Reticulum Kinase) protein. PERK is a serine/threonine-protein kinase that plays a role in the unfolded protein response.
Automatically generated - may contain errors
Lab products found in correlation
Cgrp antibody, by Abcam (1 mentions)
Ccr2 antibody, by Novus Biologicals (1 mentions)
Alexa fluor 594, by Abcam (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 conjugated donkey anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Neurotrace red, by Thermo Fisher Scientific (1 mentions)
2 protocols using perk antibody
1
Immunohistochemical Analysis of Signaling Pathways
2
Immunohistochemical Analysis of p-ERK
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The sample was fixed with 4% paraformaldehyde at 24 h post reperfusion. After dehydration with 30% sucrose, the brain was frozen and cut into 12-μm sections approximately 1.33 mm from the rostrum to the bregma. The slices were then washed with TBST, incubated with 0.3% TritonX-100 for 5 min at room temperature, and blocked with 5% fetal bovine serum and 3% BSA for 30 min. The primary antibody was rabbit against p-ERK antibody (1:200, Abcam, Cambridge, United Kingdom). After overnight incubation at 4°C, Alexa Fluor 488-conjugated donkey anti-rabbit antibody and NeuroTrace red (1:2000, Molecular Probes; a dye for staining of neurons) were used for fluorescence detection. The samples were visualized under a microscope (OLYMPUS, BX51) through the program DP2-BSW.
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