L asparagine monohydrate

Manufactured by Fujifilm

Sourced in Japan

L-asparagine monohydrate is a chemical compound that is commonly used in laboratory settings. It is a white, crystalline solid with the molecular formula C4H8N2O3·H2O. The core function of L-asparagine monohydrate is to serve as a nutrient source for certain types of microorganisms and cell cultures in various experimental and analytical applications.

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3 protocols using l asparagine monohydrate

Taste Receptor Activation by Amino Acids

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Agar (purified powder) was obtained from Sigma-Aldrich (St Louis, MO, USA). As tastants, D-fructose (CAS: 57-48-7, Wako Pure Chemical Industries, Ltd., Osaka, Japan), as well as L-alanine (ALA; CAS: 56-41-7), L-arginine monohydrochloride (ARG; CAS: 1119-34-2), L-asparagine monohydrate (ASN; CAS: 5794-13-8), L-aspartic acid (ASP; CAS: 56-84-8), L-cysteine monohydrochloride hydrate (CYS; CAS: 7048-04-6), L-histidine monohydrochloride (HIS; CAS: 645-35-2), L-isoleucine (ILE; CAS: 73-32-5), L-leucine (LEU; CAS: 61-90-5), L-lysine monohydrochloride (LYS; CAS: 657-27-2), L-glutamine (GLN; CAS: 56-85-9), L-glutamic acid (GLU; CAS: 56-86-0), L-glycine (GLY; CAS: 56-40-6), L-methionine (MET; CAS: 63-68-3), L-phenylalanine (PHE; CAS: 63-91-2), L-proline (PRO; CAS: 147-85-3), L-serine (SER; CAS: 56-45-1), L-threonine (THR; CAS: 72-19-5), L-tryptophan (TRP; CAS: 73-22-3), L-tyrosine (TYR; CAS: 60-18-4) and L-valine (VAL; CAS: 72-18-4) were used at concentrations of 10 mmol l⁻¹ (TYR was used in 0.5 mmol l⁻¹ due to low solubility). Amino acids were obtained from Wako Pure Chemical Industries, Ltd., Osaka, Japan (TYR, SER, CYS, VAL), Sigma-Aldrich, St Louis, MO, USA (ILE, PRO) or Nacalai Tesque, Kyoto, Japan (ALA, ARG, ASN, ASP, LEU, LYS, GLN, GLY, MET, PHE, THR, TRP, TYR).

Kudow N., Miura D., Schleyer M., Toshima N., Gerber B, & Tanimura T. (2017). Preference for and learning of amino acids in larval Drosophila. Biology Open, 6(3), 365-369.

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Analytical-Grade Chemical Compounds

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Propionic acid (PA), butyric acid (BA), L-glycine (Gly), L-alanine (Ala), L-serine (Ser), L-threonine (Thr), L-cysteine (Cys), L-methionine (Met), L-valine (Val), L-leucine (Leu), L-isoleucine (Ile), L-phenylalanine (Phe), L-tryptophan (Trp), L-aspartic acid (Asp), L-glutamic acid (Glu), L-asparagine (Asn), L-glutamine (Glu), L-histidine (His), L-asparagine monohydrate (Arg), L-tyrosine (Tyr), and L-ascorbic acid were purchased from FUJIFILM Wako (Osaka, Japan). Acetic acid (AA), L-lysine (Lys), and sodium butyrate were purchased from Nacalai Tesque (Kyoto, Japan), and proline (Pro) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were of analytical grade.

Takakuwa A., Nakamura K., Kikuchi M., Sugimoto R., Ohira S., Yokoi Y, & Ayabe T. (2019). Butyric Acid and Leucine Induce α-Defensin Secretion from Small Intestinal Paneth Cells. Nutrients, 11(11), 2817.

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Quantification of Amino Acids and Sugars

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Chemicals and solvents were of HPLC or analytical grade. A certified reference material, the solution containing 17 amino acids in 0.1 M HCl and the solid standard amino acids and sugars β-alanine, L-citrulline, L-ornitihine monohydrochloride, taurine, gamma-aminobutyric acid (GABA), D-fructose, xylitol and tartaric acid were acquired from Sigma Alrich (St. Louis, Missouri, USA). L-asparagine monohydrate, L-glutamine, L-tryptophan and L-theanine were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan), D-glucose and sucrose from VWR Chemicals (BDH Prolabo, Leuven Belgium) and Tri-Sil HTP Reagent was obtained from Thermo Scientific. Methanol (MeOH), acetonitrile (ACN), hydrochloric acid (35%), potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium phosphate monobasic and 3-mercaptopropionic acid (3-MPA) were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany) and VWR Chemicals (Radnor, Pennsylvania, USA). Boric acid was purchased from Merck KGaA (Darmstadt, Germany) and sodium hydroxide from VWR Chemicals (BDH Prolabo, Leuven Belgium). Derivatization reagent o-phthalaldehyde (OPA, ≥ 98%) and 85% ortophosphoric acid (85-90%) were acquired from MP Biomedicals (Santa Ana, California, USA). Ethanol anhydride was acquired from Yliopiston Apteekki (Helsinki, Finland).

Rotola-Pukkila M., Välimaa A., Suomela J., Yang B., Karhu S., & Hopia A. (2021). Effect of daily light integral treatments on free amino acids and sugars contributing flavor and acrylamide formation in potato tubers of Solanum tuberosum L. Agricultural and Food Science, 30(2).

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