Modular p800 module

The pH and PCO₂ were measured by blood gas analysis (Rapid Lab 865, Bayer Diagnostics, Tarrytown, NY, USA, at 37°C). Actual bicarbonate and base excess values were calculated according to the recommendations of the National Committee for Clinical Laboratory standards. Serum sodium, chloride, albumin and lactate concentrations were measured using central chemical analyzers (Roche Modular P800 Module, Roche Diagnostics, Indianapolis, IN, USA). We calculated the relative plasma volume changes in percentage from (Hb0/Hb90)–((1-Hct90/1-Hct0)), in which Hb is haemoglobin and Hct haematocrit, measured at 0 min and 90 min (Sysmex SE-9000, Sysmex Corporation, Kobe, Japan) [29 (link)]. We calculated, in each patient, the sodium chloride concentration difference as a measure of apparent strong ion difference and the corrected anion gap as a measure of strong ion gap [3 (link),4 (link),28 (link)]. The anion gap (AG) was calculated from sodium minus chloride and bicarbonate concentrations and the corrected AG from AG + (0.25 x (45-[albumin in g/L]))–(lactate in mmol/L) to estimate unmeasured, non-lactic acid, anions. Hence, we integratively used physochemical (according to Stewart) and Henderson-Hasselbalch principles to assess acid base status [2 (link),5 (link)].

CRP concentrations were measured using an automated turbidimetric assay (Roche Modular P800 module; Roche, Basel, Switzerland) with a lower detection limit (limit of detection, LOD) of 1 mg/L, which is routinely used in our laboratory.