Anti human cd34 fitc 8g12

Manufactured by BD

Anti human CD34 FITC (8G12) is a laboratory reagent used to detect the presence of CD34 antigen on human cells. CD34 is a surface glycoprotein found on hematopoietic stem and progenitor cells. This reagent is conjugated with the fluorescent dye FITC, allowing for the identification and quantification of CD34-positive cells through flow cytometry analysis.

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Lab products found in correlation

Anti human cd34 pe cy7, by BioLegend (2 mentions) Cd45ra bv 421, by BD (2 mentions) Cd38 apc hit2, by BD (2 mentions) Live dead fixable blue dead cell stain, by Thermo Fisher Scientific (2 mentions) Cd90 bv605, by BioLegend (2 mentions) Facsaria 2, by BD (2 mentions) Cd90 pe, by BD (1 mentions) Cd90 pe 5e10, by BD (1 mentions)

Close spelling variants of this product

CD34-FITC (134 citations) Anti-CD34 (78 citations) Anti-CD34-FITC (44 citations) Anti-human CD34-FITC (7 citations) CD34 FITC (8G12, (2 citations)

2 protocols using anti human cd34 fitc 8g12

Isolating and Modifying CD34+ HSPCs

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Harvested wells were stained with LIVE/DEAD Fixable Blue Dead Cell Stain (Life Technologies) and then with anti human CD34 PE-Cy7 (581, BioLegend), CD38 Alexa Fluor 647 (AT1, Santa Cruz Biotechnologies, Santa Cruz, CA, USA), CD45RA BV 421 (HI100, BD Biosciences), and CD90 BV605 (5E10, BioLegend) and analyzed by flow cytometry. For sorting of CD34⁺ or CD34⁺/CD38^-/CD90⁺ cells, CB-derived CD34⁺ HSPCs were stained directly after isolation from blood with anti human CD34 FITC (8G12, BD Biosciences), CD90 PE (5E10, BD Biosciences), CD38 APC (HIT2, BD Bioscience), and cells were sorted on a FACS Aria II (BD Bioscience), cultured overnight, and then electroporated with HBB RNP and transduced with HBB GFP rAAV6 using our optimized parameters.

Dever D.P., Bak R.O., Reinisch A., Camarena J., Washington G., Nicolas C.E., Pavel-Dinu M., Saxena N., Wilkens A.B., Mantri S., Uchida N., Hendel A., Narla A., Majeti R., Weinberg K.I, & Porteus M.H. (2016). CRISPR/Cas9 Beta-globin Gene Targeting in Human Hematopoietic Stem Cells. Nature, 539(7629), 384-389.

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Isolation and Characterization of Hematopoietic Stem Cells

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Cells were stained with LIVE/DEAD Fixable Blue Dead Cell Stain (Life Technologies) and then with anti-human CD34 PE-Cy7 (581, BioLegend; 1:100), CD38 Alexa Fluor 647 (AT1, Santa Cruz Biotechnologies; 1;50), CD45RA BV 421 (HI100, BD Biosciences; 1:25), and CD90 BV605 (5E10, BioLegend; 1:30) and analyzed by flow cytometry. For sorting of CD34⁺ or CD34⁺ CD38⁻ CD90⁺ cells, cord-blood-derived CD34⁺ HSPCs were stained directly after isolation from blood with anti-human CD34 FITC (8G12, BD Biosciences; 1:100), CD90 PE (5E10, BD Biosciences; 1:50), CD38 APC (HIT2, BD Bioscience; 1:50), and cells were sorted on a FACS Aria II (BD Bioscience).

Baik R., Cromer M.K., Glenn S.E., Vakulskas C.A., Chmielewski K.O., Dudek A.M., Feist W.N., Klermund J., Shipp S., Cathomen T., Dever D.P, & Porteus M.H. (2024). Transient inhibition of 53BP1 increases the frequency of targeted integration in human hematopoietic stem and progenitor cells. Nature Communications, 15, 111.

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