Mass spectrometry of protein samples were undertaken following established procedures. Thirty microliters of membrane vesicle (MV) sample and matrix-associated protein were concentrated by SDS-PAGE to obtain lanes of 5 mm width. The lanes containing proteins were subjected to in-gel trypsinolysis and proteins identified by Maxis Impact LC-QTOF mass spectrometer (Bruker, Billerica, MA, USA) using the NCBInr database and Mascot server (Matrix Science, London, UK; http://www.matrixscience.com ) to identify proteins and calculate exponentially modified protein abundance index (emPAI) values. Gene Ontology (GO) Annotation and protein subcellular locations were obtained from UniProt (www.uniprot.org ) [81 (link)].
Maxis impact lc qtof mass spectrometer
Manufactured by Bruker
Sourced in United States, Germany
The Maxis Impact LC-QTOF mass spectrometer is a high-resolution, accurate-mass instrument designed for advanced analytical applications. It combines liquid chromatography with quadrupole time-of-flight mass spectrometry to provide precise mass measurements and detailed structural information. The core function of the Maxis Impact is to perform sensitive and selective analysis of complex samples.
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Lab products found in correlation
4 protocols using maxis impact lc qtof mass spectrometer
1
Membrane Vesicle Proteome Profiling
2
Synthesis and Characterization of N-Protected Anilines
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N-Protected anilines
have been prepared using the literature procedure with slight modification
and characterized by 1H and 13C NMR.34 (link)−36 (link) Other chemicals were purchased and used directly. The 1H and 13C NMR (δ in ppm) spectra were obtained from
either a Bruker AVANCE 400 or 500 MHz spectrometer. The NMR spectra
were recorded in CDCl3 solutions with CDCl3 as
an internal lock, and TMS was used as an internal standard for 1H NMR. Mass spectra were recorded using a Bruker maXis impact
LC-q-TOF mass spectrometer. Gas chromatography–mass spectrometry
(GC–MS) analyses were carried out with an Agilent 7890A GC
system connected with a 5975C inert XL EI/CI MSD. TEM of the samples
was performed with a JEM 2100 and CM 200.
have been prepared using the literature procedure with slight modification
and characterized by 1H and 13C NMR.34 (link)−36 (link) Other chemicals were purchased and used directly. The 1H and 13C NMR (δ in ppm) spectra were obtained from
either a Bruker AVANCE 400 or 500 MHz spectrometer. The NMR spectra
were recorded in CDCl3 solutions with CDCl3 as
an internal lock, and TMS was used as an internal standard for 1H NMR. Mass spectra were recorded using a Bruker maXis impact
LC-q-TOF mass spectrometer. Gas chromatography–mass spectrometry
(GC–MS) analyses were carried out with an Agilent 7890A GC
system connected with a 5975C inert XL EI/CI MSD. TEM of the samples
was performed with a JEM 2100 and CM 200.
3
Synthesis and Characterization of Gadolinium Chelates
4
Mass Spectrometric Analysis of Oligosaccharides
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Matrix-assisted laser desorption/ionization mass spectra (MALDIMS) and electrospray ionization mass spectra (ESIMS) of obtained oligosaccharides were recorded with an Ultra Flex III MALDI-TOF/TOF mass spectrometer (Bruker, Bremen, MA, Germany) and with a Maxis Impact LC Q-TOF mass spectrometer (Bruker, Bremen, MA, Germany), respectively, in the modes described by us earlier [10 (link),26 (link)].
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