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Human magnetic luminex assay

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The Human Magnetic Luminex Assay is a multiplex immunoassay platform that allows for the simultaneous measurement of multiple analytes in a single sample. The assay utilizes magnetic beads coated with specific capture antibodies to capture and detect target analytes in a sample. The assay is designed to provide quantitative results for a range of human-specific proteins, cytokines, and other biomolecules.

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Lab products found in correlation

Luminex magpix instrument, by DiaSorin (2 mentions) Luminex magpix, by DiaSorin (1 mentions) Elisa, by R&D Systems (1 mentions) Luminex, by Merck Group (1 mentions) Enzymatic colorimetric kit, by Randox (1 mentions) Xponent 3, by DiaSorin (1 mentions) Human thrombopoietin quantikine elisa kit, by R&D Systems (1 mentions)

7 protocols using human magnetic luminex assay

1

Plasma IL-6 Quantification by Luminex Assay

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The plasma level of IL-6 was determined using a human Magnetic Luminex Assay (Bio-Techne, Abingdon, Oxon, UK) and a Magpix Luminex instrument (Luminex Corp, Austin, Texas, US) according to the manufacturer’s instruction.
Boßlau T.K., Wasserfurth P., Reichel T., Weyh C., Palmowski J., Nebl J., Joisten N., Belen S., Schenk A., Hahn A., Zimmer P, & Krüger K. (2023). 12-week combined strength and endurance exercise attenuates CD8+ T-cell differentiation and affects the kynurenine pathway in the elderly: a randomized controlled trial. Immunity & Ageing : I & A, 20, 19.
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2

Luminex-based Protein Quantification

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Medium was sampled from the OrganoPlate and added to the pre-ordered plate containing the analyte-specific capture antibodies, which bind to the analytes of interest according to the kit protocol. The Human Magnetic Luminex® Assay was used (bio-techne, #LXSAHM-11). Samples were analyzed on the MAGPIX xPONENT® software. The samples were normalized and compared to standards.
Geyer M., Gaul L.M., D`Agosto S.L., Corbo V, & Queiroz K. (2023). The tumor stroma influences immune cell distribution and recruitment in a PDAC-on-a-chip model. Frontiers in Immunology, 14, 1155085.
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3

Comprehensive Metabolic and Cytokine Profiling

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Levels of glucose, insulin, and lipid parameters were determined using standard methods in certified laboratories. FFA and glycerol levels were measured using enzymatic colorimetric kits (Randox, Crumlin, United Kingdom). Cytokines in plasma, LAT fluid and conditioned media were assessed by ELISA (Duosets, R&D Systems, Abingdon, United Kingdom) and/or Luminex (High-Sensitivity T cell panel, Merck-Millipore, Germany and Human Magnetic Luminex Assay, Bio-Techne, Minneapolis, MN, USA).
Koc M., Wald M., Varaliová Z., Ondrůjová B., Čížková T., Brychta M., Kračmerová J., Beranová L., Pala J., Šrámková V., Šiklová M., Gojda J, & Rossmeislová L. (2021). Lymphedema alters lipolytic, lipogenic, immune and angiogenic properties of adipose tissue: a hypothesis-generating study in breast cancer survivors. Scientific Reports, 11, 8171.
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4

Multiplex Cytokine Profiling in Plasma

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Plasma levels of various cytokines, chemokines and adipokines were determined using a human Magnetic Luminex Assay (Bio-Techne, Abingdon, Oxon, UK) and a Magpix Luminex instrument (Luminex Corp, Austin, TX, USA) according to manufacturer’s instructions. The following parameters were determined with the corresponding assay ranges: CC-chemokine ligand (CCL) 2 (30.9–7500 pg/mL), CCL13 (4.94–1200 pg/mL), intercellular adhesion molecule 1 (ICAM-1) (7000–1,700,000 pg/mL), IL-1ra (28.8–7000 pg/mL), IL-2 (0.64–28.00 pg/mL), IL-6 (0.85–35.00 pg/mL), IL-8 (1.6–11.70 pg/mL), IL-10 (0.24–10.0 pg/mL), IL-18 (18.5–4500 pg/mL), leptin (494–120,000 pg/mL), resistin (53.5–13,000 pg/mL), TNF-α (8.23–2000 pg/mL) and vascular endothelial growth factor (VEGF) (8.23–2000 pg/mL). All measured values for the cytokines were within the assay range and the corresponding standard curve.
Despeghel M., Reichel T., Zander J., Krüger K, & Weyh C. (2021). Effects of a 6 Week Low-Dose Combined Resistance and Endurance Training on T Cells and Systemic Inflammation in the Elderly. Cells, 10(4), 843.
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5

Multiplex Biomarker Profiling in Plasma

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Plasma levels of IL-1β, IL1ra, IL-6, IL-15, TNF-α, CX3CL1/fractalkine, adiponectin, and resistin were determined using a human Magnetic Luminex Assay (Bio-Techne, Abingdon, Oxon, UK) and a Magpix Luminex instrument (Luminex Corp, Austin, TX, USA) according to the manufacturer’s instructions.
Boßlau T.K., Wasserfurth P., Krüger B., Reichel T., Palmowski J., Nebl J., Weyh C., Schenk A., Joisten N., Stahl F., Thoms S., Gebhardt K., Hahn A, & Krüger K. (2021). Abdominal Obesity-Related Disturbance of Insulin Sensitivity Is Associated with CD8+ EMRA Cells in the Elderly. Cells, 10(5), 998.
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6

Cytokine profiling of HIV antigen stimulation

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PBMCs from healthy HIV-negative donors were resuspended at a concentration of 1 × 106/mL and were stimulated with 100μL of supernatant of gp160 (either WT or S375W)-expressing cells treated or not with temsavir (10 μM) or BNM-III-170 (50 μM). After stimulation, cells were centrifuged and the supernatants were collected. Triplicates of supernatant were analyzed using a customized Human Magnetic Luminex Assay (Bio-Techne, Minneapolis, MN, USA) to detect the following cytokines: IL-1β, IL-6, IL-10, IL-18, IL-23, IL-27, TNF-α, TSLP, CCL2, CCL4, CCL20, CXCL2 and CXCL13.
Orihuela C.J., Mills J., Robb C.W., Wilson C.J., Watson D.A, & Niesel D.W. (2001). Streptococcus pneumoniae PstS Production Is Phosphate Responsive and Enhanced during Growth in the Murine Peritoneal Cavity. Infection and Immunity, 69(12), 7565-7571.
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7

Comprehensive Serum Cytokine Analysis

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Serum cytokines were analyzed using a “Human Magnetic Luminex assay” (Bio-techne, Minneapolis, Minnesota, US) with 22 analytes: APRIL, BAFF, CCL2, CCL3, CD40L, CD138, CXCL9, CXCL10, IL-1β, IL-2, IL-4 IL-6, IL-10, IL-12, IL-13, IL-17, IL-18, IL-21, IL-33, IFN-α, IFN-γ, and TNF. Frozen patient serum samples were thawed and diluted before the experiment with an equal amount of dilution buffer and the experiment was performed according to the manufacturer’s instructions. All samples were measured on a Lifematch Fluoroanalyzer (Tepnel Life Science PLC, Wythenshawe, UK) equipped with XPonent 3.1 Software (Luminex Corporation, Austin, Texas, US). Serum TPO was quantified by ELISA using Human Thrombopoietin Quantikine ELISA Kit (R&D Systems, Minneapolis, Minnesota, US) according to manufacturer’s protocol.
Bernardes J.P., Mishra N., Tran F., Bahmer T., Best L., Blase J.I., Bordoni D., Franzenburg J., Geisen U., Josephs-Spaulding J., Köhler P., Künstner A., Rosati E., Aschenbrenner A.C., Bacher P., Baran N., Boysen T., Brandt B., Bruse N., Dörr J., Dräger A., Elke G., Ellinghaus D., Fischer J., Forster M., Franke A., Franzenburg S., Frey N., Friedrichs A., Fuß J., Glück A., Hamm J., Hinrichsen F., Hoeppner M.P., Imm S., Junker R., Kaiser S., Kan Y.H., Knoll R., Lange C., Laue G., Lier C., Lindner M., Marinos G., Markewitz R., Nattermann J., Noth R., Pickkers P., Rabe K.F., Renz A., Röcken C., Rupp J., Schaffarzyk A., Scheffold A., Schulte-Schrepping J., Schunk D., Skowasch D., Ulas T., Wandinger K.P., Wittig M., Zimmermann J., Busch H., Hoyer B.F., Kaleta C., Heyckendorf J., Kox M., Rybniker J., Schreiber S., Schultze J.L, & Rosenstiel P. (2020). Longitudinal Multi-omics Analyses Identify Responses of Megakaryocytes, Erythroid Cells, and Plasmablasts as Hallmarks of Severe COVID-19. Immunity, 53(6), 1296-1314.e9.
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