Ab30917

Manufactured by Abcam

Sourced in United Kingdom

Ab30917 is a purified antibody product provided by Abcam. This product is intended for use in laboratory research applications.

Automatically generated - may contain errors

Lab products found in correlation

Sirius red, by Merck Group (1 mentions) Olyvia build 10555, by Olympus (1 mentions) Sc 1050, by Santa Cruz Biotechnology (1 mentions) Ost 48, by Santa Cruz Biotechnology (1 mentions) Grp78, by Santa Cruz Biotechnology (1 mentions) Masson s trichrome, by Merck Group (1 mentions) Oil red o, by Merck Group (1 mentions) Vs120 virtual slide system, by Olympus (1 mentions) Lsm 780 nlo, by Zeiss (1 mentions) Sta 011, by Cell Biolabs (1 mentions) D0486, by Agilent Technologies (1 mentions)

2 protocols using ab30917

Histological Assessment of Hepatic Fibrosis

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Haemotoxylin and Eosin, Masson’s Trichrome and Sirius Red (Sigma-Aldrich, United States) stains were completed on 10% Buffered formalin fixed paraffin sections. Collagen content of the liver was quantified histologically using computerised quantification of picroSirius Red staining, as described previously¹⁵. Oil-Red-O (Sigma-Aldrich, USA) staining was performed on frozen OCT embedded cryosections as previously described^{38 (link)}. All sections were visualized on a slide scanner (Virtual Slide System VS120, Olympus, Tokyo, Japan) and viewed in the supplied program (OlyVIA Build 10555, Olympus, Tokyo, Japan). Briefly, for immunofluorescence staining, 4% PFA fixed frozen liver sections were dual stained with both anti-CML (1:125 dilution; ab30917; Abcam, United Kingdom) and OST48 (1:100 dilution; sc25558; Santa Cruz biotechnologies, United States). Dual staining on paraffin buffered formalin fixed sections was with GRP78 (1:50 dilution; sc1050; Santa Cruz biotechnologies, United States) and OST-48 (1:100 dilution; sc74407; Santa Cruz biotechnologies, United States).

Zhuang A., Yap F.Y., Bruce C., Leung C., Plan M.R., Sullivan M.A., Herath C., McCarthy D., Sourris K.C., Kantharidis P., Coughlan M.T., Febbraio M.A., Hodson M.P., Watt M.J., Angus P., Schulz B.L, & Forbes J.M. (2017). Increased liver AGEs induce hepatic injury mediated through an OST48 pathway. Scientific Reports, 7, 12292.

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Quantification of Advanced Glycation End-Products

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Several measurements were performed on transverse slices of skin biopsies. Immunohistochemical staining was conducted with the anti-AGE antibodies anti-CML (ab30917, Abcam, Cambridge, UK) and anti-N^δ-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine ([MG-H1], STA-011, cell biolabs, inc., NL) using goat anti-mouse IgG alkaline phosphatase (AP) conjugate ([Go-a-Mo-AP], D0486, Dako, Glostrup, DK) as chromogenic reporter. Histological localization of CML and MG-H1 was evaluated semi-quantitatively by two independent investigators (I.M.A and G.F.H.D). Hematoxylin and eosin (HE) stains were added to be able to identify individual cells.
Invasively assessed intrinsic fluorescence was measured by confocal microscopy using the ZEISS LSM 780 NLO (Zeiss, GER) and quantified by ImageJ. We used single (405nm and 440nm) and 2-photon (750nm, equivalent to 375nm) excitation, which corresponds to the excitation area of the AGE Reader.
In the second skin biopsy, including epidermis and dermis, the concentrations of CML, MG-H1 and pentosidine were assessed by ultra-performance liquid chromatography tandem mass spectrometry measurements (UPLC-MS/MS) and high-performance liquid chromatography (HPLC), respectively [20 , 21 (link), 22 (link)]. (Supplementary material).

Atzeni I.M., Boersema J., Pas H.H., Diercks G.F., Scheijen J.L., Schalkwijk C.G., Mulder D.J., van der Zee P, & Smit A.J. (2020). Is skin autofluorescence (SAF) representative of dermal advanced glycation endproducts (AGEs) in dark skin? A pilot study. Heliyon, 6(11), e05364.

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