DCs derived from GFP transgenic mice were monitored in a live cell imaging system (PerkinElmer, Massachusetts, USA), and time-lapse micrographs were tracked and analyzed using Volocity Demo software. The generated tracking data were used to plot the cell track and calculate the movement data.
Live cell imaging system
Manufactured by PerkinElmer
Sourced in United States
The live cell imaging system is an instrument designed for high-resolution, real-time observation and analysis of living cells. It provides the capability to capture and record dynamic cellular processes over extended periods of time.
Automatically generated - may contain errors
4 protocols using live cell imaging system
1
Tracking DCs from GFP Transgenic Mice
2
Tracking Cell Transfection Dynamics
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FHM cells were plated in glass-bottomed dishes (Nest, Wuxi, Jiangsu, China) and transfected with 800 ng pCICE in a six-well plate. The control group was transfected with 800 ng pCCE. The samples were collected at 12, 24, 48, and 72 h post-transfection. All of the cells on microscopic glass-bottomed dishes were fixed using 4% formaldehyde for 15 min at 37 °C, followed by incubation in 1 μg/mL Hoechst 33342 (Gibco, Carlsbad, CA, USA) for 15 min in the dark to stain the nuclei. Then, the stained cells were rinsed with phosphate-buffered saline (PBS). Images were taken with the Live Cell Imaging System (PerkinElmer, Waltham, MA, USA).
3
Wound Healing Assay for TNBC Cells
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TNBC cells were seeded in a 96-well plate in approximately 90% confluence. Then the wound was created across the monolayer using a nano scratch meter and the well using PBS twice. Different doses of AVB were added in wells, and the scratch images were photographed as 0 h control using a Live-cell imaging system (PerkinElmer, USA). Following the certain period of incubation, cell migration was photographed in the same field. Cells that had migrated to the scraped area were then analysed using Live-cell imaging system software.
4
Quantifying Cell Proliferation Dynamics
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The DTL plasmid- or control plasmid- transfected cells were cultured in a 24-well plate to reach approximately 50% confluency. Then the cells were observed using a live cell imaging system (PerkinElmer), Images were taken every half hour for 20 h. Finally, the images were analyzed using Living Image software (PerkinElmer) to evaluate the cell proliferation capacity.
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