Hygromycin b solution

Dulbecco’s modified minimal essential medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), trypsin-EDTA solution, 100× penicillin–streptomycin–l-glutamine solution, and streptavidin-HRP conjugate were purchased from Thermo Fisher (Waltham, MA). Puromycin and G418 solution were obtained from InvivoGen (San Diego, CA). Hygromycin B solution was purchased from Enzo Life Science (Farmingdale, NY). Anti-ORF57, anti-K8, and anti-K8.1 antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Anti-K-Rta antibody was described previously (55 (link)). All other chemicals were purchased from Millipore-Sigma (St. Louis, MO) unless otherwise stated.

Dulbecco’s modified minimal essential medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), Trypsin-EDTA solution, 100 X penicillin–streptomycin–L-glutamine solution were purchased from Thermo Fisher (Waltham, MA). Puromycin and G418 solution were obtained from InvivoGen (San Diego, CA). Hygromycin B solution was purchased from Enzo Life Science (Farmingdale, NY).
The following antibodies were used for CUT & RUN, immunoblotting and flow cytometry: rabbit anti-BRD4 (Cell Signaling, E2A7X), rabbit anti-RNAPII (Millipore, clone CTD4H8), rabbit anti-H3K27ac (CST, clone D5E4), rabbit anti-H3K4me3 (Cell Signaling, clone C42D8), mouse anti-β-actin (Santa Cruz 47778), rabbit anti-gp130 (CST), rabbit IgG (CST, clone DA1E). Rabbit anti-vIL-6 was a gift from Dr. Robert Yarchoan (NIH/NCI).

Dulbecco’s modified minimal essential medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), Trypsin-EDTA solution, 100× penicillin–streptomycin–l-glutamine solution, anti-RFP antibody, Alexa 647-conjugated secondary antibody, SlowFade Gold antifade reagent, and a LIVE/DEAD fixable yellow dead cell stain kit were purchased from Thermo Fisher (Waltham, MA USA). Puromycin solution and G418 solution were obtained from InvivoGen (San Diego, CA, USA). Hygromycin B solution was purchased from Enzo Life Science (Farmingdale, NY, USA). Anti-ORF57, anti-K8, and anti-K8.1 antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Anti-LANA and anti-lamin A/C antibodies were purchased from Millipore-Sigma (St. Louis, MO, USA). Anti-nucleolin antibody was purchased from Abcam (Cambridge, UK). Anti-K-Rta antibody was described previously (68 (link)). Monoclonal anti-ORF52 antibody was a generous gift from Fanxiu Zhu (Florida State University). All other chemicals were purchased from Millipore-Sigma (St. Louis, MO, USA) unless otherwise stated.