Enzymatically hydrolyzable lignin (EHL) was acquired from Hong Kong Laihe Biotechnology Co., Ltd. The hydroxyl content, the number-average molecular mass, and polydispersity of EHL are 2.67 mmol/g, 1430 g/mol, and 1.22, respectively. Doxorubicin hydrochloride was purchased from Shanghai Macklin Biochemical Co., Ltd. Purity is higher than 98%. THF of analytical grade purity was provided by Beijing Chemical Reagent Company. All other reagents were of analytical grade, and were used as obtained.
Doxorubicin hydrochloride
Manufactured by Maclin Biochemical Technology
Sourced in China
Doxorubicin hydrochloride is a cytotoxic anthracycline antibiotic used in the treatment of various types of cancer. It interferes with the replication of DNA and RNA, leading to inhibition of cell growth and division.
Automatically generated - may contain errors
Lab products found in correlation
Chemiluminescence detection kit, by Beyotime (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Radioimmunoprecipitation assay buffer ripa, by Beyotime (1 mentions)
Cell viability cytotoxicity assay kit, by Beyotime (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Iron 2 chloride tetrahydrate, by Maclin Biochemical Technology (1 mentions)
Iron 3 chloride, by Maclin Biochemical Technology (1 mentions)
Slc7a11, by Cell Signaling Technology (1 mentions)
Ncoa4, by Abcam (1 mentions)
Ferroorange, by Dojindo Laboratories (1 mentions)
Bodipy 581 591 c11, by Thermo Fisher Scientific (1 mentions)
Acsl4, by ABclonal (1 mentions)
Lysotracker, by Thermo Fisher Scientific (1 mentions)
2 7 dichlorodihydrofluorescein diacetate h2dcfda, by Maclin Biochemical Technology (1 mentions)
7 protocols using doxorubicin hydrochloride
1
Enzymatic Lignin Hydrolysis for Drug Delivery
2
Cytotoxicity Evaluation of Boronic Acid Conjugates
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Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), trypsin EDTA, penicillin and streptomycin and phosphate-buffered saline (PBS) were provided by Gibco (Carlsbad, CA). The cell viability/cytotoxicity assay kit, cell counting kit-8 (CCK-8), 4′, 6-diamidino-2-phenylindole (DAPI), BCA Protein Assay Kit, Coomassie brilliant blue, FITC-labelled goat anti-mouse IgG (H + L), chemiluminescence detection kit and radioimmunoprecipitation assay buffer (RIPA) were provided by Beyotime Biotechnology (Shanghai, China). 3-aminophenylboronic acid monohydrate (PBA), poly(methyl vinyl ether-alt-maleic anhydride) (PMVEMA), trithylamine, dimethyl sulfoxide (DMSO) and doxorubicin hydrochloride were obtained from Macklin Biochemical (Shanghai, China). DiD was supplied by Biotium Inc (Fremont, US). Anti-integrin α4 monoclonal antibody and anti-CCR2 monoclonal antibody were provided by Abcam (Cambridge, U.K.). Unless otherwise stated, all reagents were of analytical grade and employed without further purification.
3
Doxorubicin and YSHS Granule Biochemical Assay
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Doxorubicin hydrochloride (D807083, Macklin, China) was dissolved in 0.9% NaCl to make a 1 mg/ml solution. The YSHS granule was provided by Guangzhou Consun Pharmaceutical Co., Ltd., and was dissolved in deionized water to make a 200 mg/ml solution. Triglyceride assay kit (A110-1-1), total cholesterol assay kit (A111-1-1), creatinine assay kit (sarcosine oxidase) (C011-2-1), albumin assay kit (A028-2-1), and microalbumin assay kit (H127-1-2) were purchased from Nanjing Jiancheng Bioengineering Institute.
4
Doxorubicin-Loaded Lignin Nanocarriers
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Enzymatic hydrolysis lignin (EHL) was acquired from Hong Kong Laihe Biotechnology Co., Ltd. (Hong Kong, China) The hydroxyl content, the number-average molecular mass, and polydispersity of EHL are 2.67 mmol/g, 1430 g/mol, and 1.22, respectively. Doxorubicin hydrochloride was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China) Purity is higher than 98%. Tetrahydrofuran (THF) of analytical-grade purity was provided by Beijing Chemical Reagent Company (Beijing, China). Folic acid was supplied by Chembase Co., Ltd. (Shanghai, China) Iron (II) chloride tetrahydrate and Iron (III) chloride and albumin from bovine serum were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China) Poly (dimethyl diallyl ammonium chloride) solution (PDAC, Mw < 100,000, 35 wt.% in water, 100–200 CP (25 °C)) was provided by Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China).
5
Doxorubicin Cytotoxicity Evaluation
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Doxorubicin hydrochloride was purchased from Macklin (CAS: 25316-40-9, Shanghai, China). For Doxorubicin treatment, we prepared the Doxorubicin stocking solution at 1 mM concentration in ultrapure water. Then, different concentration of Doxorubicin (10 μΜ, 1 μΜ, 0.1 μΜ, 0.01 μΜ, 0.001 μΜ) were dissolved in medium and treated for 24 h, 48 h and 72 h and CCK8 assay was performed, and IC50 of DOX was used for treatment to identify the miRNA relative expression.
6
Ferroptosis Induction and Regulation Assay
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The antibodies GPX4 (Cat# ab125066), LC3 (Cat# ab48394), NCOA4 (Cat# ab86707) were purchased from Abcam. The antibodies SLC7A11 (Cat# 12691), P16 (Cat# 80772), P21 (Cat# 2947) were purchased from Cell Signaling Technology. The antibodies ACSL4 (Cat# A20414), FTH1 (Cat# A19544), FSP1 (Cat# A12128), DMT1/SLC11A2 (Cat# A23379), CD71/Transferrin (Cat# A21622), SLC40A1 (Cat# A14884) were purchased from Abclonal. The antibodies P62 (Cat# 18420-1-AP), GAPDH (Cat# 60004-1-lg), Ki67 (Cat# 27309-1-AP). The BODIPY™ 581/591 C11 (Cat# D3861), LysoTracker (Cat# L7526) were purchased from Thermo Fisher Scientific. The FerroOrange (Cat# F374) was purchased from Dojindo. The ferroptosis inducers, RSL3 (Cat# SML2234) was purchased from Merck; Fin56 (Cat# HY-103087) and iFSP1 (Cat# HY-136057) were purchased from MCE. The chloroquine (Cat# HY-17589A), LLOMe (Cat# HY-129905A) were purchased from MCE. Doxorubicin hydrochloride (Cat# D807083) was purchased from Macklin.
7
Synthesis of Metal-Organic Framework
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Terephthalic acid (C6H4(COOH)2, H2BDC, 98%), ferric chloride hexahydrate (FeCl3·6H2O, 98%) and polyvinylpyrrolidone K30 were obtained from Aladdin (Shanghai, China), and N,N-dimethylacetamide (DMA, 99%) was purchased from Hushi (Shanghai, China). 2′,7′-Dichlorodihydrofluorescein diacetate (H2DCFDA) and doxorubicin hydrochloride (98%) were purchased from Macklin (Shanghai, China).
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