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Rhodamineb isothiocyanate mixed isomers

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RhodamineB isothiocyanate mixed isomers is a fluorescent dye commonly used in various scientific applications. It consists of a mixture of isomeric forms of the Rhodamine B isothiocyanate compound. The core function of this product is to serve as a labeling reagent or fluorescent marker in research and analytical techniques.

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Lab products found in correlation

Polydiallyldimethylammonium chloride, by Merck Group (1 mentions) Bradford protein assay dye reagent, by Bio-Rad (1 mentions) Acetone, by Merck Group (1 mentions) Iminodiacetic acid, by Merck Group (1 mentions) Polyallylamine, by Merck Group (1 mentions) Pyridoxal 5 phosphate hydrate, by Merck Group (1 mentions) 2 phenylethylamine, by Merck Group (1 mentions) 3 aminopropyltrimethoxysilane, by Merck Group (1 mentions) Cetyltrimethylammonium bromide, by Merck Group (1 mentions) Absolute ethanol, by Merck Group (1 mentions) Iron 2 chloride tetrahydrate, by Merck Group (1 mentions) Oleic acid, by Merck Group (1 mentions) Ethyl acetate, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) Ammonium hydroxide solution, by Merck Group (1 mentions) Fluorescein isothiocyanate isomer 1, by Merck Group (1 mentions)

2 protocols using rhodamineb isothiocyanate mixed isomers

1

Agarose Microbead-Based Enzyme Assay

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Agarose microbeads (50–150 μm
diameter) were purchased from Agarose Bead Technologies (Madrid, Spain).
Polyethyleneimine (PEI) solution in H2O (Mw ∼ 60 000, 50 wt %), polyallylamine (PAH)
solution in H2O (Mw ∼
65 000, 10 wt %), polydiallyldimethylammonium chloride (PDADMAC)
solution in H2O (Mw < 100 000,
35 wt %), pyridoxal 5′-phosphate hydrate (PLP, 98%), rhodamine
B isothiocyanate mixed isomers (RhB), acetone, 2-phenylethylamine
(PEA, 98%), iminodiacetic acid (IDA), albumin bovine serum standard
(BSA), and other reagents and solvents of analytical grade were purchased
from Sigma-Aldrich (St. Louis, IL). Nicotinamide adenine dinucleotide-reduced
sodium salt (NADH) and flavin mononucleotide sodium salt (FMN) were
purchased from GERBU Biotechnik GmbH (Heidelberg, Germany). Flavin
Adenine dinucleotide disodium salt hydrate (FAD, 94%) was purchased
from Cymit Quimica S.L. (Barcelona, Spain). Riboflavin (Rf, 98%) was
purchased from Acros Organics B.V.B.A. (Fair Lawn, New Jersey, United
States). Isopropyl-β-d-thiogalactopiranoside (IPTG,
100%) was purchased from Fisher Bioreagents. The Bradford protein
assay dye reagent was purchased from BIORAD (Biorad. Hercules, CA).
Clear bottom black and white microplates (96-well) were purchased
from Avantor (2021 VWR International, LLC). μ-Slides 8 well
glass bottom was purchased from Ibidi (Planegg, Germany).
Diamanti E., Santiago-Arcos J., Grajales-Hernández D., Czarnievicz N., Comino N., Llarena I., Di Silvio D., Cortajarena A.L, & López-Gallego F. (2021). Intraparticle Kinetics Unveil Crowding and Enzyme Distribution Effects on the Performance of Cofactor-Dependent Heterogeneous Biocatalysts. ACS Catalysis, 11(24), 15051-15067.
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2

Synthesis of Fluorescent Magnetic Silica Nanoparticles

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For the synthesis of MNPSNPs, hydrophobic magnetite nanoparticles are produced first. In a second step, these particles were enveloped with a nanoporous silica shell, which in principal enables the storage of drugs, but is used here for the attachment of fluorescent dyes, namely fluorescein isothiocyanate (FITC) or rhodamine B isothiocyanate (RITC), and the modification with a PEG-silane.
For the syntheses all chemicals were used without further purification. Iron(II) chloride tetrahydrate (≥ 99%), iron(III) chloride hexahydrate (99%), oleic acid (90%), chloroform (≥ 99%), cetyltrimethylammonium bromide (CTAB, ≥ 98%), Ammonium hydroxide solution (≥ 25% NH3 in H2O) tetraethyl orthosilicate (TEOS, ≥ 99%), ethyl acetate (99.8%), 3-aminopropyl trimethoxysilane (APTMS, 97%), fluorescein isothiocyanate isomer I (FITC, ≥ 90%), rhodamine B isothiocyanate mixed isomers (RITC) were purchased from Sigma-Aldrich Corporation (München, Germany). Absolute ethanol (≥ 99.5%) was purchased from Merck (Darmstadt, Germany). [Hydroxy(polyethyleneoxy)propyl] triethoxysilane (PEG-Silane, MW 575–750 g mol−1, 50% in ethanol) was purchased from Gelest (Morrisville PA, USA). Ultrapure water (18.2 MΩ cm) was used in all chemical operations.
Janßen H.C., Warwas D.P., Dahlhaus D., Meißner J., Taptimthong P., Kietzmann M., Behrens P., Reifenrath J, & Angrisani N. (2018). In vitro and in vivo accumulation of magnetic nanoporous silica nanoparticles on implant materials with different magnetic properties. Journal of Nanobiotechnology, 16, 96.
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