R 2 2 holey carbon film

Manufactured by Quantifoil

The Quantifoil R 2/2 holey carbon film is a specimen support for transmission electron microscopy (TEM). It consists of a carbon film with a regular array of circular holes, providing an open area for imaging specimens.

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Lab products found in correlation

Vitrobot mark 4, by Thermo Fisher Scientific (3 mentions) Cryotitan, by Thermo Fisher Scientific (2 mentions) Vitrobot mark 3, by Thermo Fisher Scientific (1 mentions) Titan krios, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Holey carbon film (4 citations) Quantifoil R 2/2 carbon film (2 citations) R 1.2/1.3 holey carbon Film (2 citations)

4 protocols using r 2 2 holey carbon film

Vitrified Thin Films for CryoTEM Analysis

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Vitrified thin films for CryoTEM analysis containing MTP₁₀-HDL nanoparticles were prepared using an automated vitrification robot (FEI Vitrobot Mark III) by plunge vitrification in liquid ethane. Before vitrification, a 200-mesh copper grid covered with a Quantifoil R 2/2 holey carbon film (Quantifoil Micro Tools GmbH) was surface plasma treated for 40 seconds using a Cressington 208 carbon coater. CryoTEM imaging was performed with the Technische Universiteit Eindhoven/FEI cryoTITAN equipped with a field emission gun (FEG), a post-column Gatan energy filter (GIF) and a post-GIF 2k × 2k Gatan charge-coupled-device camera. The microscope was operated at 300 kV acceleration voltage in zero-loss energy filtering mode at a nominal magnification of 24.000 × and at a dose rate of 4.5 electrons/A²·s with a 1s image acquisition time. Representative CryoTEM micrographs show the presence of disc shaped nanoparticles in random orientations which show the highest contrast when viewed edge on resembling a line.

Priem B., van Leent M.M., Teunissen A.J., Sofias A.M., Mourits V.P., Willemsen L., Klein E.D., Oosterwijk R.S., Meerwaldt A.E., Munitz J., Prévot G., Verschuur A.V., Nauta S.A., van Leeuwen E.M., Fisher E.L., de Jong K.A., Zhao Y., Toner Y.C., Soultanidis G., Calcagno C., Bomans P.H., Friedrich H., Sommerdijk N.A., Reiner T., Duivenvoorden R., Zupančič E., Di Martino J.S., Kluza E., Rashidian M., Ploegh H.L., Dijkhuizen R.M., Hak S., Pérez-Medina C., Bravo-Cordero J.J., de Winther M.P., Joosten L.A., van Elsas A., Fayad Z.A., Rialdi A., Torre D., Guccione E., Ochando J., Netea M.G., Griffioen A.W, & Mulder W.J. (2020). Trained immunity-promoting nanobiologic therapy suppresses tumor growth and potentiates checkpoint inhibition. Cell, 183(3), 786-801.e19.

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Cryo-TEM Imaging of Vesicles and Nanocapsules

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Vitrification of the vesicles and nanocapsules samples was carried out using a vitrification robot (FEI Vitrobot Mark IV, Hillsboro, OR, USA). All samples were prepared at room temperature and 100% humidity with blotting time of 2 s and blot force of 1. The sample (5 μL) was applied onto a grid (Quantifoil, R2/2, Holey carbon film; freshly glow-discharged prior to use at 20 mA for 60 s) without dilution. Excess of sample was blotted away with filter paper to leave a thin film on the grid before being vitrified in liquid ethane. Cryo-TEM measurements were performed on FEI Titan Krios equipped with automated sample loader and a Field Emission Gun (FEG) operating at 300 kV. Images were recorded with Falcon II camera (4 × 4) with magnification of 29,000 and pixel size of 2.873 Å.

Rusli W., Jackson A.W, & van Herk A. (2018). A Roadmap towards Successful Nanocapsule Synthesis via Vesicle Templated RAFT-Based Emulsion Polymerization. Polymers, 10(7), 774.

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Vitrified Thin Films for CryoTEM Imaging

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Vitrified thin films for CryoTEM analysis were prepared using an automated vitrification robot (FEI Vitrobot Mark IV) at 60 °C and 100% relative humidity by plunge vitrification in liquid ethane. Before vitrification the plunger pipet tips were cleaned using MilliQ water and 200-mesh copper grids covered with a Quantifoil R 2/2 holey carbon film (Quantifoil Micro Tools GmbH) were surface plasma treated for 40 s using a Cressington 208 carbon coater. CryoTEM imaging was carried out on the cryoTITAN (Thermo Fisher, previously FEI), equipped with a field emission gun (FEG), a post-column Gatan imaging filter (model 2002) and a post-GIF 2k × 2k Gatan CCD camera (model 794). The microscope was operated at 300 kV acceleration voltage in bright-field TEM mode with zero-loss energy filtering at nominal magnifications of ×6500 and ×24,000; both with a 1 s image acquisition time.

Maddala S.P., Liao W.C., Joosten R.R., Soleimani M., Tuinier R., Friedrich H, & van Benthem R.A. (2021). Chain length of bioinspired polyamines affects size and condensation of monodisperse silica particles. Communications Chemistry, 4, 160.

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Cryo-TEM Analysis of Vitrified Thin Films

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Vitrified thin films for cryo-TEM analysis were prepared using an automated vitrification robot (FEI Vitrobot Mark IV) by plunge vitrification in liquid ethane. Before vitrification, a 200-mesh copper grid covered with a Quantifoil R 2/2 holey carbon film (Quantifoil Micro Tools GmbH) was surface plasma–treated for 40 s using a Cressington 208 carbon coater. Cryo-TEM imaging was carried out on the cryoTITAN (Thermo Fisher Scientific, previously FEI), equipped with a field emission gun, a post-column Gatan imaging filter (model 2002), and a post-GIF 2k × 2k Gatan charge-coupled device camera (model 794). The microscope was operated at 300-kV acceleration voltage in bright-field TEM mode with zero-loss energy filtering at a nominal magnification of ×24,000 and a dose rate of 11.8 e⁻/Å²·s with a 1^-s image acquisition time.

van Leent M.M., Meerwaldt A.E., Berchouchi A., Toner Y.C., Burnett M.E., Klein E.D., Verschuur A.V., Nauta S.A., Munitz J., Prévot G., van Leeuwen E.M., Ordikhani F., Mourits V.P., Calcagno C., Robson P.M., Soultanidis G., Reiner T., Joosten R.R., Friedrich H., Madsen J.C., Kluza E., van der Meel R., Joosten L.A., Netea M.G., Ochando J., Fayad Z.A., Pérez-Medina C., Mulder W.J, & Teunissen A.J. (2021). A modular approach toward producing nanotherapeutics targeting the innate immune system. Science Advances, 7(10), eabe7853.

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