Rna extraction kit

Manufactured by Macrogen

Sourced in Cameroon

The RNA Extraction Kit is a laboratory tool designed for the efficient extraction and purification of RNA from various biological samples. It provides a streamlined process to isolate high-quality RNA for downstream applications such as gene expression analysis, RT-PCR, and RNA sequencing.

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Lab products found in correlation

M mlv reverse transcriptase, by Promega (2 mentions) Oligo dt 15 primer, by Promega (2 mentions) Lightcycler 480, by Roche (2 mentions) Gotaq master mix, by Promega (2 mentions) Gotaq pcr master mix, by Promega (1 mentions) Lightcycler 2, by Roche (1 mentions) M mlv reverse transcriptase and oligo dt 15 primer, by Promega (1 mentions)

Spelling variants of this product

MG Total RNA Extraction Kit (5 citations) Total RNA Extraction Kit (2 citations)

3 protocols using rna extraction kit

Rice Total RNA Extraction and qPCR Analysis

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Total RNA was extracted from rice tissues using an RNA Extraction Kit (Macrogen, South Korea) according to manufacturer instructions. First-strand cDNA was synthesized with 2 µg of total RNA in a 25 µl volume using M-MLV reverse transcriptase and oligo(dT)₁₅ primer (Promega), and diluted with 75 µl of water. The qPCR amplifications were conducted on the LightCycler 2.0 instrument (Roche Diagnostics). The 20 µl of qPCR mixture included 2 µl of the first-strand cDNA mixture, 10 µl of 2X GoTaq PCR Master Mix (Promega), and 1 µl of 10 pM primer pairs (Supplementary Table S1). The qPCR conditions were 95°C for 2 min, followed by 50 cycles at 95°C for 5 s, 59°C for 15 s, and 72°C for 10 s. The rice UBIQUITIN5 (OsUBQ5, AK061988) gene was used as an internal control for normalization. The semi-quantitative PCR was performed in a 20 µl volume containing 2 µl diluted cDNA, 1 unit Ex Taq polymerase (TaKaRa Biotechniques), and 1 µl of 10 pM primers (listed in Supplementary Table S1). The PCR program included initial denaturation at 94°C for 3 min, followed by specified cycles at 94°C for 30 sec, 55°C for 1 min, and 72°C for 40 sec, followed by a final extension at 72°C for 5 min. The PCR products were electrophoresed on a 1% agarose gel. OsUBQ5 was used as an equal loading control.

Lim C., Kang K., Shim Y., Sakuraba Y., An G, & Paek N.C. (2020). Rice ETHYLENE RESPONSE FACTOR 101 Promotes Leaf Senescence Through Jasmonic Acid-Mediated Regulation of OsNAP and OsMYC2. Frontiers in Plant Science, 11, 1096.

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Quantitative RT-PCR Analysis of Rice Transcripts

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For the RT reactions, total RNA was extracted from rice leaf blades and other tissues using an RNA Extraction Kit (Macrogen, Korea). First-strand cDNA was prepared with 2 μg total RNA using M-MLV reverse transcriptase and oligo(dT)₁₅ primer (Promega) in a total volume of 25 μl and diluted with 75 μl water. For quantitative reverse-transcription PCR (qPCR), a 20 ml mixture was prepared including first-strand cDNA equivalent to 2 μl total RNA, 10 μl 2× GoTaq master mix (Promega), 6 μl distilled water, and gene-specific forward and reverse primers (Supplementary Table 1). The qPCR was performed using a LightCycler 480 (Roche Diagnostics). Rice Ubiquitin5 (UBQ5) or GAPDH (encoding glyceraldehyde phosphate dehydrogenase) was used as an internal control. The relative expression level of each gene was calculated using the 2^−ΔΔCT method, as previously described (Livak and Schmittgen, 2001 (link)).

Piao W., Han S.H., Sakuraba Y, & Paek N.C. (2017). Rice 7-Hydroxymethyl Chlorophyll a Reductase Is Involved in the Promotion of Chlorophyll Degradation and Modulates Cell Death Signaling. Molecules and Cells, 40(10), 773-786.

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Quantitative Gene Expression Analysis in Rice

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For the reverse transcription reactions, total RNA was extracted from rice leaf blades and other tissues using an RNA Extraction Kit (Macrogen, Seoul, Korea). First-strand cDNA was prepared with 2 μg total RNA using M-MLV reverse transcriptase and oligo(dT)₁₅ primers (Promega) in a total volume of 25 μl and diluted with 75 μl water. For quantitative PCR (qPCR), a 20 μl mixture was prepared including ﬁrst-strand cDNA equivalent to 2 μl total RNA, 10 μl 2×GoTaq master mix (Promega), 6 μl distilled water, and gene-speciﬁc forward and reverse primers (Supplementary Table S1). The qPCR was performed using a LightCycler 480 (Roche Diagnostics). Rice Ubiquitin5 (UBQ5) was used as an internal control. The relative expression level of each gene was calculated using the 2^−ΔΔCT method as previously described (Livak and Schmittgen, 2001 ).

Piao W., Kim S.H., Lee B.D., An G., Sakuraba Y, & Paek N.C. (2019). Rice transcription factor OsMYB102 delays leaf senescence by down-regulating abscisic acid accumulation and signaling. Journal of Experimental Botany, 70(10), 2699-2715.

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