Pdgfrb

Manufactured by R&D Systems

Sourced in Germany

PDGFRB is a receptor tyrosine kinase that binds to platelet-derived growth factors. It plays a role in cell growth, division, and survival. PDGFRB is commonly used in research applications to study signal transduction pathways and cellular processes.

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Lab products found in correlation

Poinceau, by Merck Group (1 mentions) Western lightning ecl, by PerkinElmer (1 mentions) Nkx2 2, by Aviva Systems Biology (1 mentions) Sigmafast protease inhibitor cocktail, by Merck Group (1 mentions) Alpha tubulin, by Merck Group (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Chemostar imager, by Intas (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Histofine sab po r kit, by Nichirei Biosciences (1 mentions) Rab27a, by Cell Signaling Technology (1 mentions) Simple stain max po kit, by Nichirei Biosciences (1 mentions) Doublecortin dcx, by Santa Cruz Biotechnology (1 mentions) Smi31, by Fortrea (1 mentions) Cd163, by Abcam (1 mentions)

Close spelling variants of this product

Anti-PDGFRb mouse (clone PR7212 (2 citations)

3 protocols using pdgfrb

Western Blot Analysis of Signaling Proteins

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Western blots were generated by the semi-dry method. Protein lysates from cell lines were prepared using SIGMAFast protease inhibitor cocktail (Sigma). Proteins were transferred onto nitrocellulose membranes (Bio-Rad, München, Germany) and blocked with 5% dry milk powder dissolved in phosphate-buffered-saline buffer (PBS). The following antibodies were used: MSX1 (R & D Systems), alpha-Tubulin (Sigma), PDGFRB (R & D Systems), phospho-PDGFRB (Aviva Systems Biology, Eching, Germany), NKX2-2 (Aviva Systems Biology) and PITX1 (Abnova, Taipei, Taiwan). For loading control blots were reversibly stained with Poinceau (Sigma) and detection of alpha-Tubulin (TUBA) was performed thereafter. Secondary antibodies were linked to peroxidase for detection by Western-Lightning-ECL (Perkin Elmer, Waltham, MA, USA). Documentation was performed using the digital system ChemoStar Imager (INTAS, Göttingen, Germany). PDGFD and BMP4 were quantified in the medium by ELISA using according Quantikine ELISA kits from R & D Systems. Samples were obtained by harvesting supernatants of 1x10⁶ cells which were washed in PBS and subsequently incubated in 1 ml medium for 24 h.

Nagel S., Pommerenke C., MacLeod R.A., Meyer C., Kaufmann M., Fähnrich S, & Drexler H.G. (2019). Deregulated expression of NKL homeobox genes in T-cell lymphomas. Oncotarget, 10(35), 3227-3247.

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Immunohistochemical Analysis of Tumor Tissues

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For light microscopic analysis, tumor tissues were fixed with 3% formaldehyde, paraffin embedded, and stained with hematoxylin and eosin (H&E) using standard techniques. Antibodies used were mouse CD31 (1:100, Cell Signaling, 77699), PDGFRB (1:100, R & D Systems, BAF1042), RAB27A (1:100, Cell Signaling, 69295), SYTL2 (1:100, Santa Cruz Biotechnology, sc393847), VWF (1:100, Santa Cruz Biotechnology, sc365712), human CD31 (1:100, Abcam, ab28346), FLAG-tag (1:100, Sigma-Aldrich, F3165), aSMA (1:100, DAKO, MO851), TFE3 (1:100, Santa Cruz Biotechnology, sc5958), and NG2 (1:100, Millipore, AB5320). Heat-mediated antigen retrieval was performed in Tris-EDTA buffer at pH 6.0. Immunohistochemical staining was performed using the Simple Stain MAX-PO kit (Nichirei Bioscience), the Histofine SAB-PO (R) kit (Nichirei Bioscience).

Tanaka M., Chuaychob S., Homme M., Yamazaki Y., Lyu R., Yamashita K., Ae K., Matsumoto S., Kumegawa K., Maruyama R., Qu W., Miyagi Y., Yokokawa R, & Nakamura T. (2023). ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma. Nature Communications, 14, 1957.

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Multifaceted Analysis of Brain Lesions

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Rats were transcardially perfused with 0.9% saline, brains immediately removed and fixed in 4% paraformaldehyde. A standard paraffin block was obtained from the center of the lesion (bregma −1mm to +1mm). Every 10^th coronal section for a total of 5 sections (6µm thick) was used for immunohistochemical staining. Antibody against Von Willebrand Factor (vWF, an endothelial cell marker, 1:400; Dako), α-smooth muscle actin (αSMA, smooth muscle cell marker, mouse monoclonal IgG 1:800, Dako), ED1 (microglia/macrophages marker, 1:30; AbD Serotec), CD163 (M2 macrophage marker, 1:500, Abcam), Doublecortin (DCX, a protein expressed in migrating neurons, 1:200, Santa Cruz), SMI-31 (phosphorylated neurofilament marker, 1:1000, Covance), PDGFRa (platelet derived growth factor receptor, 1:400, Santa Cruz) and PDGFRb (1:100, R&D systems) were employed. Bielschowsky silver (BS) and luxol fast blue (LFB) staining was used to demonstrate axons and myelin respectively. Antibody against albumin (albumin-FITC, polyclonal, 1:500, Abcam) was used to demonstrate BBB leakage. Gomori One-Step Trichrome Stain was used to evaluate arteriosclerosis. For control experiments non-immune serum was substituted for the primary antibody.

Yan T., Venkat P., Chopp M., Zacharek A., Ning R., Roberts C., Zhang Y., Lu M, & Chen J. (2016). Neurorestorative responses to delayed hMSC treatment of stroke in type 2 diabetic rats. Stroke, 47(11), 2850-2858.

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