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Tnf α

Manufactured by ZellBio
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Sourced in Germany

TNF-α is a recombinant protein that represents the tumor necrosis factor alpha. It is commonly used in life science research applications.

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Lab products found in correlation

Ctrp3, by ZellBio (1 mentions) Brain derived neurotrophic factor (bdnf), by ZellBio (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions)

2 protocols using tnf α

1

Blood Biomarker Sampling Protocol

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After a 12 h overnight fast, samples (5 mL) were collected from the cubital vein using standard techniques. Blood samples were collected at baseline and 48 h after the last training session. Following completion of blood collection, samples were centrifuged at 3000 rpm for 20 min, and the serum was stored at −70 °C for future analysis of adiponectin, CTRP3, CTRP5, CTRP9, TNF-α, and CRP (ZellBio GmbH, Lonsee, Germany).
Bagheri R., Kargarfard M., Jalali K., Ashtary-Larky D., Cheraghloo N., Ghobadi H., Moghadam B.H., Wong A., Nordvall M, & Dutheil F. (2023). The Effects of 12 Weeks of Concurrent and Combined Training on Inflammatory Markers, Muscular Performance, and Body Composition in Middle-Aged Overweight and Obese Males. Nutrients, 15(6), 1482.
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2

Biomarker Quantification in Brain Tissue

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Furthermore, brain tissue samples were provided and located in a cold buffer (0.1 M phosphate-buffered saline, pH 7.4, involving protease inhibitor cocktail (Roche)); for 15 minutes, it was homogenized and centrifuged at 3500 rpm, and supernatants were gathered. TNF-α (Zellbio, Germany), C-reactive protein (CRP) (Zellbio, Germany), BDNF (Zellbio, Germany), total antioxidant capacity (TAC) (Zellbio, Germany), and total oxidant capacity (TOC) (Zellbio, Germany) were measured utilizing special kits.
Gorgij E., Fanaei H., Yaghmaei P., Shahraki M.R, & Mirahmadi H. (2021). Treadmill Exercise during Pregnancy Decreased Vulnerability to Neonatal Hypoxia-Ischemia through Reducing Inflammation and Increasing Antiapoptotic Gene Expressions and Antioxidant Capacity in Rats. Stroke Research and Treatment, 2021, 5512745.
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