Anti mouse igg1 fitc

Manufactured by Southern Biotech

Anti-mouse IgG1-FITC is a fluorescently-labeled secondary antibody used for the detection of mouse IgG1 antibodies in various immunoassays and cell-based applications.

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Close spelling variants of this product

FITC-conjugated goat anti-mouse IgG1 Goat anti-mouse IgG1 FITC IgG1-FITC Mouse IgG1

3 protocols using anti mouse igg1 fitc

Synovial Cell Immunophenotyping by Flow Cytometry

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The following antibodies and reagents were used for the analysis of synovial cells with flow cytometry and cell sorting: anti-CD45-APC-H7 (2D1, BD Pharmingen), anti-CD235a-APC-Alexa Fluor750 (11E4B-7-6(KC16), Beckman Coulter), anti-CD31-PE-Cyanine7 (WM-59, eBioscience), anti-CD146-BV450 (P1H12, BD Horizon), anti-CD34-PE (4H11, eBioscience), anti-PDPN-PerCP-eFluor710 (NZ-1.3, eBioscience), anti-THY1-FITC (5E10, BD Pharmingen), anti-cadherin-11-biotin (23C6), human TruStain FcX (BioLegend), streptavidin-APC (Jackson ImmunoResearch), Live/Dead fixable aqua dead cell stain kits (Molecular Probes). For immunofluorescence staining of synovial tissue, following antibodies and reagents were used: anti-CD45 (135-4C5, AbD Serotec), anti-CD34 (EP373Y, Abcam), anti-PDPN (NZ-1.3, eBioscience), anti-THY1 (F15-42-1, Merck Millipore, and clone Thy-1A1, R&D Systems), anti-cadherin-11-Biotin (23C6), anti-Ki67 (16A8, BioLegend), anti-mouse IgG1-FITC (Southern Biotech), anti-mouse IgG2a FITC (Southern Biotech), anti-mouse IgG2b-Alexa Fluor 647 (Life Technologies), anti-rat IgG-Alexa Fluor 594 (Life Technologies), anti-rat IgG-Alexa Fluor 647, anti-rabbit IgG-Alexa Fluor 546 (Life Technologies), Hoechst 33258 (Life Technologies), and anti-FITC Alexa Fluor 488 (Life Technologies).

Mizoguchi F., Slowikowski K., Wei K., Marshall J.L., Rao D.A., Chang S.K., Nguyen H.N., Noss E.H., Turner J.D., Earp B.E., Blazar P.E., Wright J., Simmons B.P., Donlin L.T., Kalliolias G.D., Goodman S.M., Bykerk V.P., Ivashkiv L.B., Lederer J.A., Hacohen N., Nigrovic P.A., Filer A., Buckley C.D., Raychaudhuri S, & Brenner M.B. (2018). Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis. Nature Communications, 9, 789.

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Immunohistochemical Analysis of Zebrafish Hearts

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IHC of embryonic zebrafish hearts for quantification of cardiomyocytes was performed as previously described^{38 (link)}. Primary antibodies used were: Amhc (Atrial myosin heavy chain; University of Iowa Developmental Studies Hybridoma Bank) and Living Colors® polyclonal anti-DsRed antibody (Clontech). Secondary antibodies used were anti-mouse IgG1-FITC (Southern Biotech) and anti-rabbit IgG-TRITC (Southern Biotech). A Zeiss M2BioV_12 Stereo microscope was used to image hearts. Additional antibody information is included in Supplementary Table 2.

Gafranek J.T., D’Aniello E., Ravisankar P., Thakkar K., Vagnozzi R.J., Lim H.W., Salomonis N, & Waxman J.S. (2023). Sinus venosus adaptation models prolonged cardiovascular disease and reveals insights into evolutionary transitions of the vertebrate heart. Nature Communications, 14, 5509.

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Subcellular Localization of Zebrafish VDACs

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HeLa cells were transfected with a C-terminally flag-tagged zebrafish VDAC1 or VDAC2 in plasmid pCS2+ using Lipofectamine 2000 (Invitrogen). After staining with MitoTracker Orange (Invitrogen) cells were fixed in 3.7% formaldehyde and permeabilized with acetone. Immunostaining was performed using primary antibody ANTI-FLAG M2 (Sigma Aldrich, St. Luis, MO) at 1:100 and secondary antibody Anti-Mouse IgG1-FITC (Southern Biotechnology Associates, Birmingham, AL) at 1:200. Cells were mounted and counterstained using Vectashield Hard Set with DAPI (Vector Laboratories, UK).

Shimizu H., Schredelseker J., Huang J., Lu K., Naghdi S., Lu F., Franklin S., Fiji H.D., Wang K., Zhu H., Tian C., Lin B., Nakano H., Ehrlich A., Nakai J., Stieg A.Z., Gimzewski J.K., Nakano A., Goldhaber J.I., Vondriska T.M., Hajnóczky G., Kwon O, & Chen J.N. (2015). Mitochondrial Ca2+ uptake by the voltage-dependent anion channel 2 regulates cardiac rhythmicity. eLife, 4, e04801.

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