Menzel glaser polysine slides

Manufactured by Thermo Fisher Scientific

Menzel-Glaser Polysine slides are microscope slides coated with a positively charged poly-L-lysine solution. The coating enhances the adhesion of biological samples to the slide surface, improving the attachment of cells, tissues, or other biological materials during microscopy and other laboratory procedures.

Automatically generated - may contain errors

Lab products found in correlation

Dako autostainer plus, by Agilent Technologies (1 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions) 780 nlo point scanning confocal microscope, by Zeiss (1 mentions)

Spelling variants of this product

Polysine slides (41 citations) Menzel-Glaser (16 citations) Polysine (6 citations)

2 protocols using menzel glaser polysine slides

Inflammatory and Myofibroblast Marker Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD45 (leukocyte common antigen), a marker commonly used to detect inflammation, was used.^{43 (link)} To detect CD45, 5-µm sections were placed on Menzel-Glaser Polysine slides (Thermo Scientific, Waltham, Mass.) and stained using an automated system (DAKO Autostainer Plus, Dako, CO) at room temperature.
α-smooth muscle actin (α-SMA), a common marker used to detect differentiated myofibroblasts,²⁰ was used to identify these cells. α-SMA was detected in 5-µm sections that were attached to Menzel-Glaser Polysine slides (Thermo Scientific) and stained at room temperature using an automated system (DAKO Autostainer Plus).

Frenkiel B.A., Temple-Smith P., de Kretser D, & Southwick G.J. (2017). Follistatin and the Breast Implant Capsule. Plastic and Reconstructive Surgery Global Open, 5(3), e1258.

+ Open protocol

+ Expand

Quantifying γH2AX Foci in HSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

WT and Jak2^+/VF LT-HSCs (LSK+ CD48− CD150+) and MPPs (LSK+ CD48+) were isolated 48 h post treatment with 50,000 IU IFN-α or vehicle (0.1% BSA/PBS). Cells were resuspended in 30 μl PBS and placed onto polylysine slides (Thermo scientific, Menzel-Glaser Polysine slides). Cells were fixed for 15 min with 4% PFA/PBS; washed 2 × 5 min 4 °C PBS then 2 × 5 min room temperature PBS; permeabilized for 10 min 0.2% Triton/PBS; washed 2 × 5 min PBS; blocked 1 h PBS/1% FCS/0.1% Triton-X-100 at room temperature; stained γH2AX in PBS/1% FCS at 4 °C; washed 2 × 5 min PBS/1% FCS/0.1% Triton-X-100 (Sigma-Aldrich). Cells were then mounted with Prolong Gold antifade reagent with Dapi (Molecular probes Life Technologies). Cells were imaged using the Zeiss 780-NLO Point Scanning Confocal microscope. Foci were enumerated manually and a minimum of 40 cells were counted per sample. Cells with greater than three foci were considered as having greater γH2AX expression compared with baseline.

Austin R.J., Straube J., Bruedigam C., Pali G., Jacquelin S., Vu T., Green J., Gräsel J., Lansink L., Cooper L., Lee S.J., Chen N.T., Lee C.W., Haque A., Heidel F.H., D’Andrea R., Hill G.R., Mullally A., Milsom M.D., Bywater M, & Lane S.W. (2019). Distinct effects of ruxolitinib and interferon-alpha on murine JAK2V617F myeloproliferative neoplasm hematopoietic stem cell populations. Leukemia, 34(4), 1075-1089.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!