Cells were fixed with 4 % PFA and stained with a mouse anti-MRC-1 (Abcam) and a goat-anti mouse Alexa Fluor 488 secondary antibody (Life Technologies). For intracellular staining, cells were fixed with 4% PFA and permeabilized with ice cold methanol. Cells were stained with phospho-STAT1 (Tyr 701) Alexa Fluor 488 conjugated rabbit antibody (Cell Signalling), rabbit anti-SOCS1 (ab135718) and assessed by flow cytometry using the Gallios Flow Cytometer. In addition, the following antibodies from Biolegend were used in this study : CD3 (100235), CD4 (100405), CD8a (100707), CD11b (101207), CD19 (152403), CD45 (103111), CD69 (104507), CD115 (135523), CD206 (MRC-1) (141707), F4/80 (123107), FOXP3 (126403), GATA3 (653805), Gr-1 (108411), MARCO (BioRad ED31), RORγt (654301), T-bet (644809). Antibodies were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), PE-Cy7 or allophycocyanin (APC) and appropriate isotype controls were included in all experiments.
Gallios flow cytometer
Manufactured by Cell Signaling Technology
The Gallios Flow Cytometer is a high-performance flow cytometry system designed for advanced cell analysis. It provides accurate and reliable data acquisition, processing, and analysis capabilities. The Gallios Flow Cytometer is capable of simultaneous detection and measurement of multiple parameters on individual cells or particles passing through a focused laser beam.
Automatically generated - may contain errors
Lab products found in correlation
F4 80, by Bio-Rad (2 mentions)
Mouse anti mrc 1, by Abcam (2 mentions)
Phospho stat1 tyr 701 alexa fluor 488 conjugated rabbit antibody, by Cell Signaling Technology (2 mentions)
Gata3, by Bio-Rad (2 mentions)
Alexa fluor 488 goat anti mouse secondary antibody, by Thermo Fisher Scientific (2 mentions)
Cd11b, by Bio-Rad (2 mentions)
2 protocols using gallios flow cytometer
1
Multiparametric Immune Profiling by Flow Cytometry
2
Multiparametric Immune Profiling by Flow Cytometry
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Cells were fixed with 4 % PFA and stained with a mouse anti-MRC-1 (Abcam) and a goat-anti mouse Alexa Fluor 488 secondary antibody (Life Technologies). For intracellular staining, cells were fixed with 4% PFA and permeabilized with ice cold methanol. Cells were stained with phospho-STAT1 (Tyr 701) Alexa Fluor 488 conjugated rabbit antibody (Cell Signalling), rabbit anti-SOCS1 (ab135718) and assessed by flow cytometry using the Gallios Flow Cytometer. In addition, the following antibodies from Biolegend were used in this study : CD3 (100235), CD4 (100405), CD8a (100707), CD11b (101207), CD19 (152403), CD45 (103111), CD69 (104507), CD115 (135523), CD206 (MRC-1) (141707), F4/80 (123107), FOXP3 (126403), GATA3 (653805), Gr-1 (108411), MARCO (BioRad ED31), RORγt (654301), T-bet (644809). Antibodies were conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), PE-Cy7 or allophycocyanin (APC) and appropriate isotype controls were included in all experiments.
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