The viability of EC cells was detected by MTT assay. The transfected Ishikawa and HEC-1A cells were seeded into a 96-well plate (2 × 105 cells per well). Subsequently, the cells were incubated for 24, 48, 72 and 96 h, followed by adding 20 µL MTT (GENECHEM, Inc, Shanghai, China) to each well to incubate another 2 h at 37°C. The viability (OD450) was analyzed using a Multiskan Spectrum microplate reader (Thermo Fisher Scientific).
Methyl thiazolyl tetrazolium (mtt)
Manufactured by Genechem
Sourced in China
MTT is a colorimetric assay used to measure cell metabolic activity. It utilizes the yellow tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), which is reduced to purple formazan crystals by metabolically active cells. The amount of formazan produced is proportional to the number of viable cells, providing a quantitative assessment of cell viability and proliferation.
Automatically generated - may contain errors
6 protocols using methyl thiazolyl tetrazolium (mtt)
1
MTT Assay for Cell Viability
2
Cell Viability Assay Using MTT
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MTT was purchased from Genechem (Shanghai, China). The MTT assay was used to measure cell vitality. At the prescribed time points, the Ti foils that had cells were gently rinsed with PBS and then transferred to a new 24-well plate. Then, 100 μL of the MTT solution (5 mg/mL) was added into a 24-well plate, and the samples were thereafter incubated at 37°C for 4 hours. Later, 500 μL of dimethyl sulfoxide was used to dissolve formazan, and the optical density (OD) was measured at 490 nm on the microplate reader.
3
MTT Assay for Cell Viability
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The viability of EC cells was detected by MTT assay. Cells were seeded into a 96-well plate with 2 × 105 cells per well. Subsequently, the cells were incubated for 24, 48, 72 and 96 h. Then, 20 µL MTT (GENECHEM, Inc, Shanghai, China) was added to each well at different time points. After that, the cells were incubated for another 2 h at 37°C. The viability (OD450) was analysed using a Multiskan Spectrum microplate reader (Thermo Fisher Scientific).
4
Evaluating SCLC Cell Viability and Chemoresistance
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The viability of SCLC cells was detected by MTT assay. The transfected SCLC cell lines were seeded into a 96-well plate at 2×105 cells per well. Subsequently, the cells were incubated for 24, 48, 72, and 96 h, followed by adding 20 µL of MTT (GENECHEM, Inc, Shanghai, China) to each well for incubation 2 h at 37℃. For the measurement of DDP chemoresistance in SCLC cells, increasing doses of DDP (0, 0.625, 1.25, 2.5, 5, 10, 20, and 40 µM) were used to treat the transfected SCLC cells. Viability (OD450) was determined using a microplate reader (Thermo Fisher Scientific, Waltham, MA, USA).
5
MTT Assay for PC-12 Cell Viability
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The viability of PC-12 cells was detected by MTT assay. The OGD/R-induced PC-12 cells were seeded into a 96-well plate with 2 × 105 cells per well incubating for 24 h at 37°C, followed by adding 20 μl MTT (GENECHEM, Inc, Shanghai, China) to each well. After 2 h of incubation at 37°C, the viability (OD450) was analyzed by a Multiskan Spectrum microplate reader (Thermo Fisher Scientific, Waltham, MA, USA).
6
MTT Assay for Cell Viability
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The HG-induced HGMCs cells were seeded into a 96-well plate with 2 × 105 cells per well at 37°C. After incubation for 24 h, 20 µl MTT (GENECHEM, Inc, Shanghai, China) was added to each well to incubate for another 2 h at 37°C. The viability (OD450) was analyzed using a Multiskan Spectrum microplate reader (Thermo Fisher Scientific, Waltham, MA, U.S.A.).
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