Sucrose, resveratrol, and β-piceid were purchased from Sigma-Aldrich (USA). Water, methanol, and acetonitrile (high-performance liquid chromatography grade) were purchased from Burdick & Jackson (USA) for purification of transfer products. Dulbecco’s modified Eagle medium (DMEM) was purchased from WelGENE Inc. (Korea). Fetal bovine serum (FBS) and antibiotics (penicillin/streptomycin) were purchased from Gibco (Thermo Fisher Scientific, USA). Diphenyl-2-picrylhydrazyl (DPPH) and 2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) were purchased from Sigma-Aldrich. All other chemicals were of reagent grade and purchased from Sigma-Aldrich. The recombinant amylosucrase from D. geothermalis (DGAS) was prepared in E. coli as previously described [16 (link)].
Antibiotics penicillin streptomycin
Manufactured by Thermo Fisher Scientific
Sourced in United States
Penicillin/streptomycin is a commonly used antibiotic mixture for cell culture applications. It is a sterile liquid solution containing the antibiotics penicillin and streptomycin.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (12 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (3 mentions)
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Glutamax, by Thermo Fisher Scientific (3 mentions)
L glutamine, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Trizol, by Thermo Fisher Scientific (3 mentions)
Glutamine, by Thermo Fisher Scientific (3 mentions)
Propidium iodide, by Merck Group (2 mentions)
Iscript cdna synthesis kit, by Bio-Rad (2 mentions)
Faststart universal sybr green master rox, by Roche (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Methanol, by Avantor (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Dulbecco s modified eagle medium dmem, by Welgene (1 mentions)
28 protocols using antibiotics penicillin streptomycin
1
Amylosucrase-Catalyzed Synthesis of Sucrose Derivatives
2
Apoptosis Regulation Mechanisms in Cell Lines
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18α-Glycyrrhetinic acid (18α-GA), 4,6-diamidino-2-phenylindole (DAPI), dimethyl sulfoxide (DMSO), N-acetyl-l -cysteine (NAC), propidium iodide (PI) and trypsin-EDTA were obtained from Sigma Chemical Co. (St. Louis, MO, USA). RPMI-1640 medium, fetal bovine serum (FBS), l -glutamine and antibiotics (penicillin-streptomycin) were purchased from GIBCO®/Invitrogen Life Technologies (Carlsbad, CA, USA). Primary antibody against PARP, caspase-3,-8, -9, AIF, Endo G, Bid, Bax, Bcl-2, Bcl-xL, cytochrome c, Fas, Fas-L and peroxidase conjugated secondary antibodies were purchased from Cell Signaling (St Louis, MO, USA). 18α-GA was dissolved in DMSO. Cell culture grade DMSO was used for vehicle at 0.1%.
3
Chitosan Immunomodulatory Effects Evaluation
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Acetic acid, concanavalin A (Con A), dimethyl sulfoxide (DMSO), lipopolysaccharide (LPS) and YAC-1 cells were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). RPMI-1640 medium, fetal bovine serum (FBS), L-glutamine and antibiotics (penicillin-streptomycin) were purchased from Gibco; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Antibodies against CD3, CD19, CD11b and Mac-3 were obtained from BD Biosciences (San Jose, CA, USA). Chitosan powder with molecular weight of ~86,000 kDa (Koyo Chemical Co., Ltd, Osaka, Japan) was obtained from the National Taiwan University College of Medicine Animal Medicine Center (Taipei, Taiwan, Republic of China). It was dissolved in Acetic acid, and 5 and 20 mg/kg solutions were separately suspended in 0.2 ml Acetic acid at room temperature for 1 h before use (20 (link)).
4
Optimized Gelatin-Based Biomaterial Synthesis
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Gelatin from porcine skin, type A (300 bloom, MW: 50~100 k); Gelatin from bovine skin, type B (~225 bloom, MW: 40~50 k); gallic acid with commercial reference G7384, having 97.5–102.5% (titration); 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC); N-hydroxysuccinimide (NHS); and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma (St. Louis, MO, USA). Quercetin dihydrate and dimethyl sulfoxide (DMSO) were obtained from Merck (Darmstadt, Germany). High-glucose Dulbecco’s modified eagle medium (HG-DMEM), foetal bovine serum, trypsin-EDTA and antibiotics (penicillin/streptomycin, 200 U/mL) were purchased from GIBCO (Grand Island, NY, USA). The total RNA purification mini kit was from FavorPrep, and the iScriptTM cDNA Synthesis Kit was from Bio-Rad (Hercules, CA, USA). FastStart Universal SYBR Green Master (ROX) was purchased from Roche (Mannheim, Germany). Polydimethylsiloxane (PDMS) SYLGARD® 184 was obtained from Dow Corning. Purified water (18.2 MΩ cm at 25 °C) was from Direct-Q1 ultra-pure water system, Millipore, Billerica, MA, USA. All other chemicals and solvents were of analytical grade and were used without further purification.
5
Isolation and Culture of Corneal Stromal Stem Cells
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CSSCs were isolated from corneas of three donors aged 15 to 45 years old, as described in Supplementary Table S1 and cultured in a stem cell growth medium (SCGM)15 (link) consisting of Dulbecco's modified Eagle's medium (DMEM) low glucose, MCDB-201, insulin-transferrin-selenious acid supplement (Sigma-Aldrich, St. Louis, MO, USA), gentamicin, albumin, fetal bovine serum (FBS) (2%) (Gibco, Gaithersburg, MD, USA), ascorbic acid-2-phosphate, dexamethasone, platelet-derived growth factor, epidermal growth factor, and antibiotics penicillin/streptomycin (Gibco). Cells were cryopreserved in liquid nitrogen using 70% DMEM/HAMs F12, 20% FBS, and 10% dimethyl sulfoxide (DMSO) for 10 years or more. After 10 years, CSSCs were quickly thawed using water bath maintained at 37°C and cultured in SCGM. TrypLE express (Gibco) was used for cell detachment and further upscale. Cells were routinely observed under bright field inverted microscope (Nikon Eclipse TS100; Melville, NY, USA) and medium was changed every third day.
6
Cell Signaling Pathway Analysis
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TET, dimethyl sulfoxide (DMSO) and propidium iodide were obtained from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Leibovitz's L-15 medium, fetal bovine serum (FBS), L-glutamine and antibiotics (penicillin-streptomycin) were purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Primary and secondary antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Polyvinylidene difluoride (PVDF) membrane was obtained from EMD Millipore (Billerica, CA, USA).
7
MG-63 Osteosarcoma Cell Culture Protocol
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MG-63 osteosarcoma cell line was acquired from the National Cell Bank of Institute Pasteur of Iran. The cell line was cultured according to the supplier's recommendation, in Dulbecco's Modified Eagle Medium (DMEM), supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco) and 1% antibiotics (penicillin/streptomycin) (Gibco) and incubated in a humidified 37°C incubator with 5% CO2. DMEM media was substituted with fresh medium every 3–4 days. For subculturing, cells were harvested by Trypsin/EDTA(Gibco) treatment.
8
Engineered Hydrogel Microenvironment
9
Apoptosis Induction in A549 Lung Cancer Cells
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A549 human lung cancer cells were obtained from the Korean Cell Line Bank (Seoul, Korea). RPMI-1640 medium, fetal bovine serum (FBS) and antibiotics (penicillin/streptomycin) were purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Hoechst 33342 and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Materials and chemicals used for electrophoresis were obtained from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). Antibodies against Bcl-xL (#2762), Bax (#2772), caspase-3 (#9662), caspase-6 (#9762), caspase-8 (#9746) and caspase-9 (#9502), cleaved caspase-3 (#9661), poly (adenosine diphosphate-ribose) polymerase (PARP; #9542) and cleaved PARP (#9541), were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Anti-cyclin B1 (#05-373), anti-cyclin-dependent kinase 1 (CDK1; #06-923), anti-cell division cycle 25c (cdc25c; #05-507) and anti-β-actin antibodies (#MABT825) were obtained from EMD Millipore (Billerica, MA, USA). Horseradish peroxidase (HRP)-coupled goat anti-mouse IgG ALX-211-205TS-C100 and anti-rabbit IgG ADI-SAB-301-J were purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). Muse™ Annexin V & Dead Cell kit was purchased from EMD Millipore.
10
MT-4 Cell Line Cultivation Protocol
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The human T lymphoblastoid cell line MT-4 (HTLV-1 transformed) was cultured in RPMI 1640 complete medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Sigma, USA), 1% GlutaMAX (Gibco, USA) and 1% antibiotics penicillin-streptomycin (Gibco, USA). Cells were passaged three times a week and cultured at a density of <0.5×106 cells/mL.
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