Plko 1 puro tfam

Manufactured by Merck Group

PLKO.1-puro-Tfam is a plasmid vector used for the expression and purification of the transcription factor A, mitochondrial (Tfam) protein in mammalian cell lines. The vector contains a puromycin resistance gene for selection of stable cell lines expressing the Tfam protein.

Automatically generated - may contain errors

Lab products found in correlation

Plko 1 puro ddr1, by Merck Group (2 mentions) Plko 1 puro sdc1, by Merck Group (2 mentions) Plko 1 puro nrf2, by Merck Group (2 mentions) Plvx ires puro nrf2e79q flag, by Sangon (2 mentions)

Spelling variants of this product

PLKO.1-puro (99 citations) PLKO.1 (97 citations) ShRNAs in the pLKO.1-puro vector for TFAM (2 citations)

2 protocols using plko 1 puro tfam

CRISPR-Cas9 and Lentiviral Knockdown Protocols

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

For gene ablations, the target cDNA sequences (Supplementary Table 1) of mouse Ddr1, Mrc2, Itgb1, Lair1, Nrf2, Col1a1, and human DDR1 were cloned into lentiCRISPR v2-Blast vector or lentiCRISPR v2-puro vector, respectively using BsmBI. For gene knockdowns (KD), pLKO.1-puro-Ddr1 (TRCN0000023369), pLKO.1-puro-Ddr1 (TRCN0000121163), pLKO.1-puro-Sdc1 (TRCN0000302270), pLKO.1-puro-Nrf2 (TRCN0000054658) and pLKO.1-puro-Tfam (TRCN0000086064) were ordered from Sigma. pCDH-CMV-MCS-EF1-puro-Col1α1-6*His and pLVX-IRES-Puro-NRF2^E79Q-Flag were made by Sangon Biotech (Shanghai, China). pLKO.1-blast-Ikkα, pLKO.1-puro-Nhe1, pLKO.1-puro-NHE1, pLKO.1-puro-Nrf2, and lentiCRISPR v2-Puro-p62/Sqstm1 were described^{11 (link)}. LentiCRISPR v2-Blast-ATG7^{30 (link)} was a gift from Dr. Sina Ghaemmaghami.

Su H., Yang F., Fu R., Trinh B., Sun N., Liu J., Kumar A., Baglieri J., Siruno J., Le M., Li Y., Dozier S., Nair A., Filliol A., Sinchai N., Rosenthal S.B., Santini J., Metallo C.M., Molina A., Schwabe R.F., Lowy A.M., Brenner D., Sun B, & Karin M. (2022). Collagenolysis-dependent DDR1 signalling dictates pancreatic cancer outcome. Nature, 610(7931), 366-372.

+ Open protocol

+ Expand

Gene Ablation and Knockdown Protocols

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For gene ablations, the target cDNA sequences (Supplementary Table 1) of mouse Ddr1, Mrc2, Itgb1, Lair1, Nrf2, Col1a1 and human DDR1 were cloned into a lentiCRISPR v2-Blast vector or lentiCRISPR v2-puro vector, respectively using BsmBI. For gene knockdowns, pLKO.1-puro-Ddr1 (TRCN0000023369), pLKO.1-puro-Ddr1 (TRCN0000121163), pLKO.1-puro-Sdc1 (TRCN0000302270), pLKO.1-puro-Nrf2 (TRCN0000054658) and pLKO.1-puro-Tfam (TRCN0000086064) were ordered from Sigma. pCDH-CMV-MCS-EF1-puro-Col1α1-6XHis and pLVX-IRES-Puro-NRF2^E79Q-Flag were made by Sangon Biotech (Shanghai, China). pLKO.1-blast-Ikkα, pLKO.1-puro-Nhe1, pLKO.1-puro-NHE1, pLKO.1-puro-Nrf2, and lentiCRISPR v2-Puro-p62/Sqstm1 have been described previously¹¹. LentiCRISPR v2-Blast-ATG7 (ref. ^{29 (link)}) was a gift from S. Ghaemmaghami.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!