In GCKD, rs41272110 was represented directly on the genotyping microarray. In KORA F3 and F4 genotypes were retrieved from Haplo-type Reference Consortium-imputed data [34 (link)] using the Imputation Server [35 (link)] (imputation quality for KORA F3: R2 = 0.96953 and KORA F4: R2 = 0.95874). Genotyping arrays were Illumina Omni2.5Exome array for GCKD, Illumina Omni2.5 and Illumina Omni Express for KORA F3 and Affymetrix Axiom for KORA F4 with call rates >98%. KIV-2 4925G>A carrier status had been determined earlier by amplification-refractory TaqMan PCR (described in [10 (link),14 (link)]).
Omni express
Manufactured by Thermo Fisher Scientific
The Omni Express is a versatile lab equipment designed for sample preparation and processing. It features a compact and durable construction with the capability to handle a wide range of sample volumes. The core function of the Omni Express is to efficiently homogenize and disrupt samples to extract target analytes or components for further analysis.
Automatically generated - may contain errors
3 protocols using omni express
1
Genotyping Protocols for Genetic Study
2
Genotyping Protocols for Genetic Study
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In GCKD, rs41272110 was represented directly on the genotyping microarray. In KORA F3 and F4 genotypes were retrieved from Haplo-type Reference Consortium-imputed data [34 (link)] using the Imputation Server [35 (link)] (imputation quality for KORA F3: R2 = 0.96953 and KORA F4: R2 = 0.95874). Genotyping arrays were Illumina Omni2.5Exome array for GCKD, Illumina Omni2.5 and Illumina Omni Express for KORA F3 and Affymetrix Axiom for KORA F4 with call rates >98%. KIV-2 4925G>A carrier status had been determined earlier by amplification-refractory TaqMan PCR (described in [10 (link),14 (link)]).
3
Evaluating CNVR Probe Coverage
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In order to evaluate the coverage by array probes for all CNVR sets, we used bedtools (v2.29.2) [67 (link)] intersect to determine all markers falling within a respective CNVR and then counted the number of markers, reporting zero for no intersection. For this we used array probe maps from several chip designs from [4 (link)]: Illumina’s OmniExpress, CytoSNP850, Omni25, Omni5 as well as Affymetrix SNP 6.0 and CytoScan HD
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