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Env 223am

Manufactured by Med Associates

The ENV-223AM is a laboratory equipment designed for environmental monitoring and analysis. It provides accurate and reliable measurements of various environmental parameters.

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3 protocols using env 223am

1

Multimodal Feedback System for Behavioral Studies

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A pair of desktop computer speakers were placed in the bottom of the frame to provide auditory feedback. A pellet dispenser (ENV-203-1000; Med-Associates, St. Albans, VT, USA) was connected to the receiving raspberry pi via the PCB, located across the room. A Sonalert tone generator (ENV-223AM; 2900 Hz, 100 dB; Med-Associates) was also attached alongside the pellet dispenser.
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2

Rat Operant Food Conditioning Protocol

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Before the first self-administration training session, we gave the rats a 1-h magazine-training session, which began with the presentation of the white house light, followed by the noncontingent delivery of one pellet every 3 min. We used 45-mg food pellets (12.7% fat, 66.7% carbohydrate, and 20.6% protein; TestDiet 45-mg pellet, catalog #1811155). We then trained the rats to lever press for food during six 1-h sessions that were separated by 10 min for six consecutive days. The sessions began with the presentation of the white house light, followed 10 s later by the insertion of the food-paired active lever (right panel). The white house light remained on for the duration of the session and served as a discriminative cue for the palatable food. We trained the rats under a fixed-ratio-1 (FR1) 20-s timeout reinforcement schedule, where one lever press resulted in the delivery of five 45-mg palatable food pellets and the presentation of a 20-s discrete tone cue (ENV-223AM, Med Associates), during which additional lever presses were not reinforced but still recorded. At the end of each 1-h session, the white house light was turned off and the active lever was retracted. To match the number of discrete cue presentations to that of fentanyl (see below), we limited the number of food-reinforced deliveries to 12/h.
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3

Operant Behavior Testing Platform for Neuroscience Research

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The operant behavior testing (OBT) components were obtained from Med Associates, Inc. (Fairfax, VT): nose poke (ENV-114AM), cue light (ENV-221M), tone generator (ENV-223AM), pellet dispenser (ENV-203) and pellet receptacle (ENV-200R2M). Lithium-Ion batteries (12 V, 10 Ah Model: CR12V10Ah with BMS protection circuit) were purchased from Dakota Lithium Battery (Seattle, WA). An MD-ROOC uses one battery at a time. A bidirectional digital I/O interface (NI 9401), wireless data acquisition unit (cDAQ 9191) and interface software (LabVIEW 2016) originated from National Instruments (Austin, TX). The wireless-LAN router (E1200 N300 Wi-Fi Router) was from Linksys (Irvine, CA). The battery delivers the power for all OBT and National Instruments components. A locally constructed digital interface contains an in-house built printed circuit board that accepts digital inputs to control MedAssociates components as well as receiving signals from the nose poke. (Supplementary Information Figures S.13).
Ordinarily, OBT componentsuse 28 V for both power and control signals. However, they can be configured, or modified, to use 12 V signals instead. Table 1 lists the modifications neccessary to allow the OBT components to be operated at 12 V.
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