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Mx cre

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Mx-Cre is a Cre recombinase gene that is inducible by type I interferons. It is commonly used in genetically modified mouse models to achieve conditional gene expression or deletion in a temporal and tissue-specific manner.

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Lab products found in correlation

Poly 1 c, by Merck Group (1 mentions) Vav cre, by Jackson ImmunoResearch (1 mentions) Polyinosinic polycytidylic acid pipc, by Merck Group (1 mentions)

2 protocols using mx cre

1

Generation of Conditional Knockout Mice

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The generation of Gata3f/f mice is described elsewhere (26 (link)). Gata3f/f mice were back-crossed to either Mx-Cre (Jackson Laboratory, Bar Harbor, ME) (40 (link)) or Vav-Cre transgenic mice [(41 (link)); a kind gift from Dr. Nancy Speck, University of Pennsylvania]. To allow for an inducible deletion of the Gata3 floxed alleles, Gata3f/f-Mx-Cre+ mice were injected with 400 μg of poly (I:C) i.p. 3 times (Sigma-Aldrich) over 6 days. Cdkn2bf/f mice (42 (link)) were obtained from the NCI mouse repository (Frederick, MD), and backcrossed with Gata3f/f Vav-Cre+ transgenic mice to obtain double-conditional knockout dcKO (Cdkn2bf/f;Gata3f/f;Vav-Cre+) mice. Bone marrow was harvested 14 to 30 days later. All animal procedures were approved by the Sunnybrook Health Science Centre Animal Care Committee.
Thompson P.K., Chen E.L., de Pooter R.F., Frelin C., Vogel W.K., Lee C.R., Venables T., Shah D.K., Iscove N.N., Leid M., Anderson M.K, & Zúñiga-Pflücker J.C. (2022). Realization of the T-lineage program involves GATA-3 induction of Bcl11b and repression of Cdkn2b expression. Journal of immunology (Baltimore, Md. : 1950), 209(1), 77-92.
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2

Conditional Deletion of hnRNP L in Mice

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hnRNP L floxed mice were described previously26 (link). MxCre, VavCre, H2kBcl2 and Trp53−/− mice were obtained from Jackson laboratories or from a colony maintained at the IRCM. The animal ethics committee of the IRCM approved all animal experiments. MxCre+hnRNP Lfl/fl or hnRNP Lfl/fl mice were injected intraperitoneally with 500 μg of polyinosinic-polycytidylic acid (pIpC; Sigma-Aldrich) every other day for a total of 5 times and were analyzed 14 days after the first injection. All mice were aged between 8–16 weeks for BM analysis.
Gaudreau M.C., Grapton D., Helness A., Vadnais C., Fraszczak J., Shooshtarizadeh P., Wilhelm B., Robert F., Heyd F, & Möröy T. (2016). Heterogeneous Nuclear Ribonucleoprotein L is required for the survival and functional integrity of murine hematopoietic stem cells. Scientific Reports, 6, 27379.
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