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100mm mercaptoethanol

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100mM-mercaptoethanol is a reducing agent used in various laboratory procedures. It helps maintain the reduced state of proteins and other biomolecules by preventing oxidation.

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Lab products found in correlation

Esgro mlif, by Merck Group (2 mentions) B27 supplement, by Thermo Fisher Scientific (2 mentions) Fibroblast growth factor 2 (fgf2), by Thermo Fisher Scientific (2 mentions) 96 well ultra low adhesion culture plates, by Corning (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) N2 supplement, by Thermo Fisher Scientific (2 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions)

2 protocols using 100mm mercaptoethanol

1

Pancreatic Tumorsphere Assay for Tumor and Circulating Cells

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(A) Pancreatic tumorsphere formation assays were performed on freshly isolated mouse pancreatic tumor cells or circulating tumor cells from peripheral blood modified from Rovira, et al32 (link). Briefly, pancreatic tumors from 10-13 week old REM1-KPf/fC or REM2-KPf/fC mice were dissociated and FACS sorted for YFP+ and YFP- or EpCAM+/GFP+ and EpCAM+/GFP- cells, respectively. 100-500 cells were suspended in 100μl DMEM F-12 (Gibco, Life Technologies) containing 1x B-27 supplement (Gibco, Life Technologies), 3% FBS, 100mM-mercaptoethanol (Gibco, Life Technologies), 1x non-essential amino acids (Gibco, Life Technologies), 1x N2 supplement (Gibco, Life Technologies), 20ng/ml EGF (Gibco, Life Technologies), 20ng/ml FGF2 (Gibco, Life Technologies), and 10ng/ml ESGRO mLIF (Millipore). Culture media for circulating tumor cells also contained 20ng/ml mHGF (R&D Systems). Cells in media were plated in 96-well ultra-low adhesion culture plates (Costar) and incubated at 37°C for 7 days. Sphere images were obtained with a Nikon80i. Sphere size was measured using ImageJ 1.47v software.
Fox R.G., Lytle N.K., Jaquish D.V., Park F.D., Ito T., Bajaj J., Koechlein C.S., Zimdahl B., Yano M., Kopp J., Kritzik M., Sicklick J., Sander M., Grandgenett P.M., Hollingsworth M.A., Shibata S., Pizzo D., Valasek M., Sasik R., Scadeng M., Okano H., Kim Y., MacLeod A.R., Lowy A.M, & Reya T. (2016). Image based detection and targeting of therapy resistance in pancreatic adenocarcinoma. Nature, 534(7607), 407-411.
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2

Pancreatic Tumorsphere Assay for Tumor and Circulating Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
(A) Pancreatic tumorsphere formation assays were performed on freshly isolated mouse pancreatic tumor cells or circulating tumor cells from peripheral blood modified from Rovira, et al32 (link). Briefly, pancreatic tumors from 10-13 week old REM1-KPf/fC or REM2-KPf/fC mice were dissociated and FACS sorted for YFP+ and YFP- or EpCAM+/GFP+ and EpCAM+/GFP- cells, respectively. 100-500 cells were suspended in 100μl DMEM F-12 (Gibco, Life Technologies) containing 1x B-27 supplement (Gibco, Life Technologies), 3% FBS, 100mM-mercaptoethanol (Gibco, Life Technologies), 1x non-essential amino acids (Gibco, Life Technologies), 1x N2 supplement (Gibco, Life Technologies), 20ng/ml EGF (Gibco, Life Technologies), 20ng/ml FGF2 (Gibco, Life Technologies), and 10ng/ml ESGRO mLIF (Millipore). Culture media for circulating tumor cells also contained 20ng/ml mHGF (R&D Systems). Cells in media were plated in 96-well ultra-low adhesion culture plates (Costar) and incubated at 37°C for 7 days. Sphere images were obtained with a Nikon80i. Sphere size was measured using ImageJ 1.47v software.
Fox R.G., Lytle N.K., Jaquish D.V., Park F.D., Ito T., Bajaj J., Koechlein C.S., Zimdahl B., Yano M., Kopp J., Kritzik M., Sicklick J., Sander M., Grandgenett P.M., Hollingsworth M.A., Shibata S., Pizzo D., Valasek M., Sasik R., Scadeng M., Okano H., Kim Y., MacLeod A.R., Lowy A.M, & Reya T. (2016). Image based detection and targeting of therapy resistance in pancreatic adenocarcinoma. Nature, 534(7607), 407-411.
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