LPS was purchased from Sigma-Aldrich (St. Louis, MO, USA) and diluted in pyrogen-free 0.9% saline. Insulin was obtained from Sigma-Aldrich (USA). TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, USA). Anti-human APN, anti-Nnat, anti-peroxisome proliferator-activated receptor (PPAR) γ, anti-p65, and anti-inhibitor of nuclear factor κB (IκB) α were purchased from Abcam (Cambridge, MA, USA). Anti-β-actin antibody was purchased from Jiancheng Bioengineering Institute of Nanjing (Nanjing, JS, China). Reactive oxygen species (ROS), total antioxidant capacity (T-AOC), malondialdehyde (MDA), and superoxide dismutase (SOD) detection kits were purchased from Jiancheng Bioengineering Institute of Nanjing (Nanjing, JS, China).
Superoxide dismutase sod detection kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Superoxide Dismutase (SOD) Detection Kit is a laboratory equipment designed to measure the activity of the antioxidant enzyme superoxide dismutase. The kit provides a simple and reliable method for the quantitative determination of SOD levels in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti nnat, by Abcam (1 mentions)
Anti p65, by Abcam (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Insulin, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Neutral gum, by Solarbio (1 mentions)
Enhanced chemiluminescence ecl kit, by Bio-Rad (1 mentions)
Polyvinylidene fluoride pvdf membrane, by Merck Group (1 mentions)
Bicinchoninic acid bca kit, by Beyotime (1 mentions)
Female balb c mice, by Shanghai Model Organisms (1 mentions)
Male c57bl 6 mice, by Shanghai Model Organisms (1 mentions)
Lipopolysaccharide, by Merck Group (1 mentions)
Malondialdehyde mda, by Nanjing Jiancheng (1 mentions)
Paraffin, by Solarbio (1 mentions)
T aoc detection kit, by Nanjing Jiancheng (1 mentions)
Ab32135, by Abcam (1 mentions)
Hrp labeled goat anti mouse igg h l, by Beyotime (1 mentions)
Anti β actin antibody, by Beyotime (1 mentions)
Lipid peroxidation malonaldehyde mda assay kit, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
7 protocols using superoxide dismutase sod detection kit
1
Insulin and Lipopolysaccharide-Induced Inflammation
2
Investigating Inflammatory Responses in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male C57BL/6 mice and female BALB/c mice were purchased from Shanghai Model Organisms Center Inc. Lipopolysaccharide and TUNEL kits were purchased from Sigma, USA. In total, 10% chloral hydrate was purchased from Zhujiang Hospital of Southern Medical University. Polyvinylidene fluoride (PVDF) membranes were purchased from Millipore, USA. Protein primary antibodies were purchased from cell signaling technology, USA. Protein secondary antibody and bicinchoninic acid (BCA) kits were purchased from Shanghai Beyotime Biotechnology Co., Ltd. Enhanced chemiluminescence (ECL) kit was purchased from Bio-Rad, USA. EPO (product batch number: 20,100,901, product specification 5000 IU/branch) was purchased from China resources Angde Biotech Pharma Co., Ltd. 4% paraformaldehyde was purchased from Guangzhou Chemical Reagent Factory (GCRF). Paraffin, xylene, and neutral gum were purchased in Beijing Solarbio Science & Technology Co., Ltd. SDS-PAGE gel preparation kit and RIPA lysate were purchased from Wuhan Servicebio Co., Ltd. Malondialdehyde (MDA) and superoxide dismutase (SOD) detection kits were purchased from Nanjing Jiancheng Bioengineering Institute.
3
Antioxidative Capacity Assessment of hfPMSC-CMs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Total Antioxidative Capacity (T-AOC) Detection Kit, Hydroxyl Free Radical (·OH) Detection Kit, Superoxide Anion Free Radical (O) Detection Kit, Superoxide Dismutase (SOD) Detection Kit, and Glutathione Peroxidase (GSH-PX) Detection Kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China). The DPPH Kit for free radical scavenging ability was purchased from Sigma-Aldrich (St. Louis, MO, USA). All these kits were used to detect the antioxidative capacity of hfPMSC-CMs from different passages.
4
Molecular Mechanisms of Podocyte Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SAA was provided by Shandong Target Drug Research Co. Ltd (Shandong, China). Prednisone acetate was the product of Zhejiang Xianju Pharmaceutical Co. Ltd. (Zhejiang, China). Doxorubicin hydrochloride for injection was produced by Shenzhen Main Luck Pharmaceuticals Inc. (Shenzhen, China). The antibodies used in this study were anti-NF-κB p65 (ab16502, Abcam), anti-inhibitor of NF-κB (IκB) α (ab32135, Abcam), anti-phosphorylation-IκBα (p-IκBα, Ser-36, ab133462, Abcam) and anti-podocin (sc-21009, Santa Cruz Biotechnology, Inc.). Goat anti-rabbit IgG was also the product of Abcam. BCA protein assay kit, anti-β-actin antibody, HRP-labeled goat anti-mouse IgG (H+L) and lipid peroxidation malonaldehyde (MDA) assay kit were obtained from Beyotime Institute of Biotechnology (Jiangsu, China). Superoxide dismutase (SOD) detection kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
5
Doxofylline Mitigates Lung Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The drugs and reagents utilized were as follows: doxofylline infusion solution (batch number: 20160115, Jiangsu Enhua Pharmaceutical Co., Ltd.); Fujian tobacco (China Tobacco Industry Co., Ltd., of Fujian Province); bacterial lipopolysaccharide (LPS) (batch number: L2880-10MG, Sigma Co., USA); rat tumor necrosis factor α (TNF-α), interleukin (IL)-8, IL-10 enzyme-linked immunosorbent assay (ELISA) test kit (Shanghai Youyou Biotechnology Co., Ltd.); malondialdehyde (MDA) detection kit A003-1 (thiobarbituric acid [TBA] method, Nanjing Jiancheng Institute of Biological Engineering); superoxide dismutase (SOD) detection kit (Nanjing Jiancheng Institute of Biological Engineering); and blood gas analysis test card (SC80 BASIC).
6
Oxidative Stress Assessment in Hyphae
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 0.5 g fresh hyphae were ground into fine powder with liquid nitrogen in a mortar. The detection of different parameters was performed by Malondialdehyde (MDA) Detection Kit (A003–1-2), Oxygen Free Radical (OFR) Detection Kit (A052–1-1), and Superoxide Dismutase (SOD) Detection Kit (A001–1-2) (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer’s instructions.
7
Astaxanthin Mitigates Rat Oxidative Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One hundred and forty-four clean, healthy rats (male and female), weighing 280±20 g, were provided by the Animal Experimental Center of Jilin University. Astaxanthin (Sigma, USA) was dissolved in olive oil immediately before use. We also obtained a malondialdehyde (MDA) detection kit, superoxide dismutase (SOD) detection kit (Nanjing Jiancheng Institute of Biological Engineering, Nanjing, China), apoptosis detection kit (Wuhan Boster Bioengineering Technology., Ltd., Wuhan, China), Motic Med 6.0 Digital Medical Image Analysis System [Motic (Xiamen) Medical Diagnostic Systems Co., Ltd., Xiamen, China], JEM-1200EX transmission electron microscope (Japan), and LKB-5 ultra-thin microtome (LKB company, Sweden). A protocol was prepared before the study without registration. Experiments were performed under a project license granted by Institutional Animal Care and Use Committee of Jilin University, in compliance with Institutional Animal Care and Use Committee of Jilin University guidelines for the care and use of animals.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!