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Anti atr

Manufactured by Proteintech
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Anti-ATR is a laboratory product offered by Proteintech. It serves as a tool for researchers to detect and study the ATR (Ataxia Telangiectasia and Rad3-related) protein, which plays a crucial role in the cellular response to DNA damage and replication stress.

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Lab products found in correlation

Phospho histone h3 ser10, by Merck Group (2 mentions) Picrosirius red, by Abcam (2 mentions) Prolong diamond antifade mountant, by Thermo Fisher Scientific (2 mentions) Anti claspin, by Thermo Fisher Scientific (2 mentions) Anti orc2, by Thermo Fisher Scientific (2 mentions) F4 80, by Abcam (2 mentions) Anti mcm2, by Abcam (2 mentions) Anti cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions) Fitc annexin 5 apoptosis detection kit with 7 aad, by BioLegend (1 mentions) Anti ddb1, by Proteintech (1 mentions) Anti cleaved caspase8 asp387, by Cell Signaling Technology (1 mentions) Anti β actin, by Proteintech (1 mentions) Bcl 6, by BD (1 mentions) Anti caspase 8, by Cell Signaling Technology (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions) Ifn γ, by BioLegend (1 mentions) Anti p53, by Proteintech (1 mentions) Enhanced chemiluminescence, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Anti dna pkcs, by Abcam (1 mentions)

Close spelling variants of this product

Anti-TM (2 citations)

4 protocols using anti atr

1

Picrosirius Red and Immunofluorescence Staining

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Picrosirius red (Abcam; Cat# ab245887) and immunofluorescence staining were performed by standard methods using 8 μm paraffin sections. Antibodies used for immunofluorescence staining were F4/80 (Abcam; Cat# ab6640; 1:100), Ly6g (Abcam; Cat# ab25377; 1:100), phospho-histone H3 Ser10 (Millipore; Cat# 06-570; 1:200), anti-Claspin (Thermofisher; Cat# PA5-102840; 1:100), anti-MCM2 (Abcam; Cat# ab4461; 1:100), anti-Orc2 (Thermofisher; (Cat# PA5-70227; 1:100), and anti-ATR (Proteintech; Cat# 19787-1-AP; 1:100).
Sections were blocked in 3% BSA-PBST, and antibodies were diluted using 1% BSA-PBST. Primary antibodies were incubated overnight at 4 °C. Secondary antibodies were incubated for 1 h at room temperature. Slides were stained with DAPI during washing and coverslips mounted with ProLong Diamond Antifade Mountant (Invitrogen; Cat# P36961).
Xiao S., Liang R., Lucero E., McConnell B.K., Chen Z., Chang J., Navran S., Schwartz R.J, & Iyer D. (2022). STEMIN and YAP5SA synthetic modified mRNAs regenerate and repair infarcted mouse hearts. The journal of cardiovascular aging, 2(3), 31.
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2

Picrosirius Red and Immunofluorescence Staining

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Picrosirius red (Abcam; Cat# ab245887) and immunofluorescence staining were performed by standard methods using 8 μm paraffin sections. Antibodies used for immunofluorescence staining were F4/80 (Abcam; Cat# ab6640; 1:100), Ly6g (Abcam; Cat# ab25377; 1:100), phospho-histone H3 Ser10 (Millipore; Cat# 06-570; 1:200), anti-Claspin (Thermofisher; Cat# PA5-102840; 1:100), anti-MCM2 (Abcam; Cat# ab4461; 1:100), anti-Orc2 (Thermofisher; (Cat# PA5-70227; 1:100), and anti-ATR (Proteintech; Cat# 19787-1-AP; 1:100).
Sections were blocked in 3% BSA-PBST, and antibodies were diluted using 1% BSA-PBST. Primary antibodies were incubated overnight at 4 °C. Secondary antibodies were incubated for 1 h at room temperature. Slides were stained with DAPI during washing and coverslips mounted with ProLong Diamond Antifade Mountant (Invitrogen; Cat# P36961).
Xiao S., Liang R., Lucero E., McConnell B.K., Chen Z., Chang J., Navran S., Schwartz R.J, & Iyer D. (2022). STEMIN and YAP5SA synthetic modified mRNAs regenerate and repair infarcted mouse hearts. The journal of cardiovascular aging, 2(3), 31.
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3

Multiparametric Flow Cytometry Panel

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Fluorescence-conjugated antibodies for CD4(Cat # 48-0042-82), CD8(Cat # 47-0081-82), CD44(Cat # 11-0441-85), CD45.1(Cat # 47-0453-82), CD45.2(Cat # 48-0454-82), Streptavidin(Cat # 25-4317-82), IgD(Cat # 48-5993-82), and Ki67(Cat # 25-5698-82) were purchased from eBioscience; CXCR5(Cat # 551961), CD138(Cat # 553713), IgG2α(Cat # 553894), PD1(Cat # 562671), Bcl-6(Cat # 561525), Fas(Cat # 554258), GL7(Cat # 553666), and H2A.X(pS139)(Cat # 564719) were purchased from BD Biosciences; B220(Cat # 103212), SLAM(Cat # 115904), IFNγ(Cat # 505810), and FITC Annexin V Apoptosis detection kit with 7-AAD(Cat # 640922) were purchased from Biolegend.
Anti-Ddb1 (cat #11380-1-AP), anti-β-Actin (cat #66009-1-Ig), and anti-ATR (cat #19787-1-AP) were purchased from Proteintech; anti-p53 (cat #2524), phosphor-Chk1 (S345) (cat #2348T), anti-cleaved-caspase8 (Asp387) (cat #8592S), anti-caspase8 (cat #4790T), anti-cleaved-caspase3 (Asp175) (cat #9664S), and anti-caspase3 (cat #9662) were purchased from Cell Signaling technology (CST); anti-Prkdc(cat #SC-390849) was purchased from SantaCruz; anti-ATM (cat #A19650) and anti-Chk1 (cat #A7653) were purchased from ABclonal Technology.
Yang L., Chen W., Li L., Xiao Y., Fan S., Zhang Q., Xia T., Li M., Hong Y., Zhao T., Li Q., Liu W.H, & Xiao N. (2021). Ddb1 Is Essential for the Expansion of CD4+ Helper T Cells by Regulating Cell Cycle Progression and Cell Death. Frontiers in Immunology, 12, 722273.
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4

Western Blot Analysis of DNA Damage Response Proteins

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Cells were lysed in RIPA buffer containing 1% PMSF and phosphatase inhibitor cocktail buffer (Beyotime, China). Proteins were loaded onto SDS-PAGE gel for electrophoresis and then transferred onto a PVDF membrane. The blots were probed with primary antibodies at 4 °C overnight followed by incubation with secondary antibodies at room temperature for 1 h. The signal was visualized using enhanced chemiluminescence (Invitrogen, Carlsbad, CA, USA) and quantified by densitometry using Image Lab software. The primary antibodies were listed as follows: anti-GAPDH (Cell Signaling Technology (CST), Cat: 2118); anti-DNA-PKcs (abcam, Cat: ab70250); anti-ATM (CST, Cat: 2873); anti-ATM (Ser1981) (CST, Cat: 5883); anti-ATR (Proteintech, Cat: 19787-1-AP); anti-ATR (Ser428) (CST, Cat:2853); anti-Rad50 (CST, Cat: 3427); anti-NBS1 (Proteintech, Cat: 55025-1-AP); anti-Mre11 (CST, Cat: 4847); anti-Ku80 (Proteintech, Cat: 16389-1-AP); anti-Ku70 (Proteintech, Cat: 10723-1-AP); anti-Akt (CST, Cat: 9272); anti-Akt (Ser473) (CST, Cat: 9271); anti-Chk2 (Thr68) (CST, Cat: 2661); anti-Chk1 (Ser345) (CST, Cat: 2348); anti-PARP (CST, Cat: 9532); anti-Bcl-2 (CST, Cat:15071); anti-Bax (CST, Cat:5023); and anti-γH2AX (ABclonal, Cat: AP0099).
Guo Z., Wang Y.H., Xu H., Yuan C.S., Zhou H.H., Huang W.H., Wang H, & Zhang W. (2021). LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma. Cell Death & Disease, 12(1), 69.
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