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Stempro osteogenesis differentiation media kit

Manufactured by Thermo Fisher Scientific

The StemPro Osteogenesis Differentiation Media Kit is a cell culture medium designed to support the differentiation of stem cells into osteogenic (bone) lineages. The kit provides the necessary components to promote the formation of osteoblasts, the cells responsible for bone formation.

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2 protocols using stempro osteogenesis differentiation media kit

1

Osteoblast Differentiation Protocol

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Cells were seeded onto 6-well plates at a density of 50,000 cells/well and allowed to recover from trypsinisation for 2 days in maintenance medium. After recovery, cells were cultured in the StemPro Osteogenesis Differentiation Media Kit (Life Technologies) according to the manufacturer’s instructions. Osteoblast differentiation was evaluated by using 2 % Alizarin Red S stain (Sigma).
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2

Osteoblast Differentiation Potency Assay

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Cells were seeded onto 6-well-plates at a density of 50,000 cells/well and allowed to recover from trypsinisation for 2 d in maintenance medium. After recovery, cells were cultured in StemPro® Osteogenesis Differentiation Media Kit, Life Technologies) according to the manufacturer’s instructions. Osteoblast differentiation was evaluated using 2% Alizarin Red S stain (Sigma).
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