Strains: P. aeruginosa INCQS 0230/ATCC 27853 and two strains of P. aeruginosa acquired from hospital infections – P.a.1 and P.a.2.
Silver nanoparticles 10 nm: AgNP 20 µg/mL solution (nanoparticles, 10 nm particle size [TEM], 0.02 mg/mL in aqueous buffer, contains sodium citrate as stabilizer – 730785, Sigma-Aldrich, St. Louis, MO, USA), tested at concentrations: 5.0, 1.25, and 0.156 µg/mL.
Antibiotics discs: ceftazidime (30 µg), meropenem (10 µg), amikacin (30 µg), ampicillin + sulbactam (20 µg), levofloxacin (5 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), oxacillin (1 µg), cefoxitin (30 µg), and erythromycin (15 µg) (Laborclin, Sao Paulo, Brazil).
Cell lines: Mouse fibroblasts NCTC-929 were purchased from Adolfo Lutz Institute; HeLa and HepG2 were kindly provided by Hemocentro – USP, Ribeirao Preto; cell culture medium DME and trypsin-EDTA (Sigma-Aldrich; EUA) and fetal bovine serum (FBS, Invitrogen).
Silver nanoparticles 10 nm: AgNP 20 µg/mL solution (nanoparticles, 10 nm particle size [TEM], 0.02 mg/mL in aqueous buffer, contains sodium citrate as stabilizer – 730785, Sigma-Aldrich, St. Louis, MO, USA), tested at concentrations: 5.0, 1.25, and 0.156 µg/mL.
Antibiotics discs: ceftazidime (30 µg), meropenem (10 µg), amikacin (30 µg), ampicillin + sulbactam (20 µg), levofloxacin (5 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), oxacillin (1 µg), cefoxitin (30 µg), and erythromycin (15 µg) (Laborclin, Sao Paulo, Brazil).
Cell lines: Mouse fibroblasts NCTC-929 were purchased from Adolfo Lutz Institute; HeLa and HepG2 were kindly provided by Hemocentro – USP, Ribeirao Preto; cell culture medium DME and trypsin-EDTA (Sigma-Aldrich; EUA) and fetal bovine serum (FBS, Invitrogen).