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Mammocult basal media

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MammoCult Basal media is a serum-free and phenol red-free basal medium designed to support the growth and maintenance of mammary epithelial cells in culture. The medium is optimized to maintain the undifferentiated state of mammary stem and progenitor cells.

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Lab products found in correlation

Ultra low attachment 6 well plate, by Corning (1 mentions) 6 well ultra low attachment culture plates, by Corning (1 mentions)

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MammoCult media (36 citations) MammoCult (24 citations)

2 protocols using mammocult basal media

1

Mammosphere Formation and Passaging

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Cells were plated at a density of 2 × 104 cells/well into ultra-low attachment 6-well plates (Corning, Corning, NY, USA) in MammoCult Basal media (StemCell Technologies). Spheres with a minimum diameter of 50 μm (or >15 cells) were counted at Day 7. For serial passaging, mammospheres were collected by centrifugation at 350g, dissociated with 0.25% trypsin, counted, and re-seeded.
Davies A.H., Reipas K.M., Pambid M.R., Berns R., Stratford A.L., Fotovati A., Firmino N., Astanehe A., Hu K., Maxwell C., Mills G.B, & Dunn S.E. (2014). YB-1 transforms human mammary epithelial cells through chromatin remodeling leading to the development of basal-like breast cancer. Stem cells (Dayton, Ohio), 32(6), 1437-1450.
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2

Mammosphere Formation Assay Protocol

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Cells were washed through a 40 μm filter to obtain a single-cell suspension and seeded at 2 × 104 per well into ultra-low attachment 6-well culture plates (Corning, Corning, NY, USA) in MammoCult Basal media (StemCell Technologies, Vancouver, Canada). Spheres with a minimum diameter of 50 μm were counted at 7 days. For serial passaging, mammospheres were collected by centrifugation at 350g for 2 minutes, dissociated with 0.25% trypsin, and re-seeded.
Davies A.H., Reipas K., Hu K., Berns R., Firmino N., Stratford A.L, & Dunn S.E. (2015). Inhibition of RSK with the novel small-molecule inhibitor LJI308 overcomes chemoresistance by eliminating cancer stem cells. Oncotarget, 6(24), 20570-20577.
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