The antibodies used in this study were as follows: USP28 (17707-1-AP, 1:1000 WB, Proteintech); RECQL1 (A300-450A, 1:1000 WB, Bethyl Lab, Montgomery); WRN (4666S,1:1000 WB,Cell Signaling); BLM (A300-110A,1:1000 WB, Bethyl Lab); RECQL4 (17008-1-AP,1:1000 WB, Proteintech); RECQL5 (A302-520A, 1:2000 WB, Bethyl Lab); Flag (F3165, 1:2000, Sigma); Ub (SC-8017, 1:200 WB, Santa Cruz Biotechnology); γH2AX (05-636, 1:500 IF, Millipore); Claspin (2800S, 1:1000 WB, Cell Signaling); c-MYC (SC-40, 1:200 WB, Santa Cruz); Phospho-Chk1-Ser345 (2348, 1:1000 WB, Cell Signaling); CHK1 (ab32531, 1:1000 WB, Abcam); GAPDH (60004-1-1g, 1:5000 WB, Proteintech); Tubulin (66240-1-1g, 1:5000 WB, Proteintech); ACTIN (66009-1-1g, 1:3000 WB, Proteintech). The secondary antibodies conjugated to horseradish peroxidase for Western blot and the secondary antibodies anti-mouse, -goat or -rabbit Alexa fluor 488 or 594 for immunofluorescence staining were purchased from Jackson ImmunoResearch Laboratories. siRNAs were synthesized by GenePharma.
Usp28 17707 1 ap
Manufactured by Proteintech
Sourced in China
USP28 (17707-1-AP) is a primary antibody produced by Proteintech. It is designed to detect the expression of the USP28 protein, which is a member of the ubiquitin-specific protease (USP) family and is involved in the regulation of cellular processes such as DNA damage response and cell cycle progression.
Automatically generated - may contain errors
Lab products found in correlation
Recql1 a300 450a, by Fortis Life Sciences (2 mentions)
Wrn 4666s, by Cell Signaling Technology (2 mentions)
Blm a300 110a, by Fortis Life Sciences (2 mentions)
Ub sc 8017, by Santa Cruz Biotechnology (1 mentions)
Phospho chk1 ser345 2348, by Cell Signaling Technology (1 mentions)
Flag f3165, by Merck Group (1 mentions)
γh2ax 05 636, by Merck Group (1 mentions)
C myc sc 40, by Santa Cruz Biotechnology (1 mentions)
Gapdh 60004 1 1g, by Proteintech (1 mentions)
2 protocols using usp28 17707 1 ap
1
Antibody Characterization for Helicase Enzymes
2
Western Blot Antibodies for DNA Repair
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The antibodies used in this study were as follows: USP28 (17707-1-AP, 1:1000 WB, Proteintech, Wuhan, China); RECQL1 (A300-450A, 1:1000 WB, Bethyl Lab, Montgomery, TX, USA); WRN ( 4666S,1:1000 WB ,Cell Signaling, Beverly, MA, USA); BLM (A300-110A ,1:1000 WB, Bethyl Lab); RECQL4 (17008-1-AP ,1:1000 WB, Proteintech); RECQL5 (A302-520A, 1:2,000 WB, Bethyl Lab); Flag (F3165,
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