Hyflo Super Cel (Diatomaceous earth), 3-aminopropyl(diethoxy)methylsilane (APDMS, 97%), dimethyl suberimidate dihydrochloride (DMS, 98%), lysozyme solution (50 mg/mL in distilled water), sodium hydroxide solution (50% in H2O), N-Acetyl-L-cysteine (NALC, 99%), sodium citrate, and Triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tris-HCI (pH 8.0), distilled water (DNase/RNase-Free), and EDTA (pH 8.0) were purchased from Invitrogen (Waltham, MA, USA). Proteinase K solution (>600 mAU/mL) was purchased from Qiagen (Hilden, Germany). Absolute ethanol was purchased from Merck (Whitehouse Station, NJ, USA). Phosphate-buffered saline (PBS; 10×, pH 7.4) was purchased from Gibco (Grand Island, NY, USA).
Tris hci
Manufactured by Thermo Fisher Scientific
Sourced in United States
Tris-HCl is a buffer solution commonly used in biochemical and molecular biology applications. It provides a stable pH environment for various enzymatic reactions and biomolecular interactions. The active component, Tris (tris(hydroxymethyl)aminomethane), is a primary amine that acts as a pH buffer when combined with hydrochloric acid (HCl).
Automatically generated - may contain errors
Lab products found in correlation
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (2 mentions)
Triton x 100, by Merck Group (2 mentions)
Lysozyme solution, by Merck Group (1 mentions)
Sodium hydroxide solution, by Merck Group (1 mentions)
Dimethyl suberimidate dihydrochloride, by Merck Group (1 mentions)
N acetyl l cysteine, by Merck Group (1 mentions)
Rnase free dnase, by Thermo Fisher Scientific (1 mentions)
Hyflo super cel, by Merck Group (1 mentions)
Proteinase k solution, by Qiagen (1 mentions)
Sodium citrate, by Merck Group (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Lysozyme from chicken egg white, by Merck Group (1 mentions)
Sodium dodecyl sulfate solution, by Merck Group (1 mentions)
Ultrapure distilled water, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
2 protocols using tris hci
1
DNA Extraction Protocol using Diatomaceous Earth
2
Magnetic Bead-based Oligonucleotide Capture
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Triton X-100 (Sigma-Aldrich), Tris-HCI (1
M, pH 8.0, Invitrogen), EDTA (0.5 M, pH 8.0, Invitrogen), lysozyme
from chicken egg white (Sigma-Aldrich), streptavidin conjugated magnetic
beads (1 μm, OceanNanoTech), sodium chloride (Sigma-Aldrich),
20× SSC (IBI Scientific), sodium dodecyl sulfate solution (10%,
Sigma-Aldrich), UltraPure distilled water (Invitrogen), bovine serum
albumin (>98%, Sigma-Aldrich), 1× phosphate buffered saline
(Sigma-Aldrich),
Biotinylated single-stranded oligonucleotides with C12 linker between
biotin and oligonucleotides (Eton Bioscience).
EC1:5′-biotin-C12-CTGCGGGTAACGTCAATGAGCAAA-3′
EC2:5′-GGTATTAACTTTACTCCCTTCCTC-C12-biotin-3′.
M, pH 8.0, Invitrogen), EDTA (0.5 M, pH 8.0, Invitrogen), lysozyme
from chicken egg white (Sigma-Aldrich), streptavidin conjugated magnetic
beads (1 μm, OceanNanoTech), sodium chloride (Sigma-Aldrich),
20× SSC (IBI Scientific), sodium dodecyl sulfate solution (10%,
Sigma-Aldrich), UltraPure distilled water (Invitrogen), bovine serum
albumin (>98%, Sigma-Aldrich), 1× phosphate buffered saline
(Sigma-Aldrich),
Biotinylated single-stranded oligonucleotides with C12 linker between
biotin and oligonucleotides (Eton Bioscience).
EC1:5′-biotin-C12-CTGCGGGTAACGTCAATGAGCAAA-3′
EC2:5′-GGTATTAACTTTACTCCCTTCCTC-C12-biotin-3′.
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