Mouse anti pcna antibody

Manufactured by Agilent Technologies

The Mouse anti-PCNA antibody is a laboratory reagent used for the detection and localization of the Proliferating Cell Nuclear Antigen (PCNA) protein in various samples. PCNA is a key protein involved in DNA replication and repair processes.

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Lab products found in correlation

Rabbit anti phospho histone h3 antibody, by Merck Group (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Goat anti dcx antibody, by Santa Cruz Biotechnology (1 mentions) Fluorescein isothiocyanate fitc conjugated donkey anti goat igg, by Santa Cruz Biotechnology (1 mentions) Lsm 510 confocal laser scanning microscope, by Zeiss (1 mentions) Vectashield, by Vector Laboratories (1 mentions)

2 protocols using mouse anti pcna antibody

Quantifying Cell Proliferation and Apoptosis in Kidney

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For cell proliferation, 4-μm sections of paraffin-embedded kidneys were incubated with a rabbit anti-phospho-histone H3 antibody (Millipore) at 1:200 and a mouse anti-PCNA antibody (DAKO) at 1:50, followed by the appropriate secondary antibody. The number of proliferating cells was determined in UB and expressed as the number of pH3- or PCNA- positive cells per UB structure on whole kidney sections.
Apoptosis was detected in 4-μm sections of paraffin-embedded kidneys by TUNEL assay using the In Situ Cell Death Detection kit (Roche) according to the manufacturer’s protocol. The number of apoptotic cells was determined as the number of TUNEL–positive nuclei per microscopic field in whole kidney sections. Ten microscopic fields were scored for each kidney in 5 embryos of each genotype.

Phelep A., Laouari D., Bharti K., Burtin M., Tammaccaro S., Garbay S., Nguyen C., Vasseur F., Blanc T., Berissi S., Langa-Vives F., Fischer E., Druilhe A., Arnheiter H., Friedlander G., Pontoglio M, & Terzi F. (2017). MITF – A controls branching morphogenesis and nephron endowment. PLoS Genetics, 13(12), e1007093.

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Curcumin's Impact on Neuroblast Regeneration

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To investigate the effects of curcumin on the regeneration of neuroblasts after MPTP-induced destruction in the SVZ, double-labeling immunofluorescence histochemistry was performed using the goat anti-Dcx antibody (Santa Cruz Biotechnology) and mouse anti-PCNA antibody (Dako). Tissue sections were visualized with fluorescein isothiocyanate (FITC)-conjugated donkey anti-goat IgG (1:100; Santa Cruz Biotechnology) and Texas Red-conjugated anti-goat antibody (1:100; Vector Laboratories, Burlingame, CA, USA), mounted with Vectashield (Vector Laboratories), and then observed using a Zeiss LSM510 confocal laser scanning microscope.

He X.J., Uchida K., Megumi C., Tsuge N, & Nakayama H. (2015). Dietary curcumin supplementation attenuates 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxicity in C57BL mice. Journal of Toxicologic Pathology, 28(4), 197-206.

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