Anti cd69 biotin

Manufactured by Thermo Fisher Scientific

Anti-CD69-biotin is a biotin-conjugated monoclonal antibody that binds to the CD69 cell surface antigen. CD69 is an early activation marker expressed on the surface of various immune cell types.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using anti cd69 biotin

Multi-Marker Immunophenotyping of T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-CD3-AlexaFluor®700 (UCHT1), anti-CD4-APC-eFluor®480 (RPAT4), anti-CD69-biotin (FN50), anti-CD127-PerCP-Cy5.5 (eBioRDRS), anti-CD27-APC-eFluor®780 (LG.7F9), anti-CD4-FITC (RPA-T4), anti-CD27-PE-Cy7 (LG.7F9), anti-CD45-APC (2D1), and anti-EpCAM-PE (IB7), anti-FoxP3-PE-Cy7 (PCH101), anti-IL-2-PE-Cy7 (MQ1-17H12), anti-IFNγ-APC-eFluor®780 (4S.B3), anti-TNFα-PerCP-Cy5.5 (Mab11), anti-PD-1-APC (MIH4), anti-PD-L1-PE-Cy7 (MIH1), mIgG1κ-PE-Cy7 (P3.6.2.8.1), anti-CD45RAFITC (HI100), anti-CD45RO-biotin (UCHL1), CD62L-PE-Cy7 (DREG-56), CCR7-APC-efl780 (3D12), anti-CD3 (OKT3), and anti-CD28 (CD28.2) were purchased from eBioscience (San Diego, CA). Anti-CD8-BV510 (RPA-T8) was purchased from BioLegend (San Diego, CA). Anti-CTLA-4-Biotin and anti-Ki67-PerCP-Cy5.5 (B56) were purchased from BD Biosciences (San Diego, CA). Anti-TIM-3-PE (344823) was purchased from R&D systems. Anti-LAG-3-FITC (17B4) was purchased from Enzo Life Sciences International, Inc. (Plymouth Meeting, PA). Anti-CD107a-PE-Cy7 (H4A3) and anti-perforin-PerCP-Cy5.5 (δG9) were purchased from BD Biosciences. Anti-galectin-9 (ab69630) was purchased from Abcam. Control rabbit IgG (BA-1000) was purchased from Vector Labs (Burlingame, CA). Goat-anti-Rabbit IgG-HRP was purchased from Dako (Carpinteria, CA). All antibodies were used per the manufacturer's recommendations.

Severson J.J., Serracino H.S., Mateescu V., Raeburn C.D., C.McIntyre R J.r., Sams S.B., Haugen B.R, & French J.D. (2015). PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer immunology research, 3(6), 620-630.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell staining was performed at 4°C with Fc Block (hybridoma 2.4G2) in FACS staining buffer (0.5% FBS and 0.1% NaN₃ in dPBS). Anti–B220-FITC, anti–B220-PE, anti–CD21-APC, Annexin V-FITC, anti–BrdU-FITC, and Streptavidin-PE were purchased from BioLegend. Anti–CD23-biotin and anti–CD138-biotin were purchased from BD. Anti–CD69-biotin, anti–MHC-II-PE, and anti–TLR9-FITC were purchased from eBioscience. Flow data were acquired by the FACSCanto II (BD) and analyzed by FlowJo software.

Chen T.T., Tsai M.H., Kung J.T., Lin K.I., Decker T, & Lee C.K. (2016). STAT1 regulates marginal zone B cell differentiation in response to inflammation and infection with blood-borne bacteria. The Journal of Experimental Medicine, 213(13), 3025-3039.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!