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Discovery v8 axio cam mrc

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The Discovery V8/Axio Cam MRC is a microscope system from Zeiss. It provides high-quality imaging capabilities for a variety of applications. The system combines the Discovery V8 microscope and the Axio Cam MRC camera, enabling detailed observation and documentation of specimens.

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Lightsheet z 1, by Zeiss (2 mentions) Axiovision le software, by Zeiss (1 mentions)

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Discovery V8 (122 citations)

3 protocols using discovery v8 axio cam mrc

1

Fluorescent Embryo Imaging and Cell Counting

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Fluorescent embryos were treated as above and imaged by fluorescence microscopy using a Zeiss Discovery V8/Axio Cam MRC and Axiovision LE software (Carl Zeiss), as previously described [34 (link)]. Cell counts were quantified using ImageJ (NIH). Two-tailed Student's t-tests were performed for investigational comparisons; one-tailed tests were run for statistical confirmation of WISH phenotypes. Data are presented as mean ± SEM, and p-values < 0.05 were considered significant.
Lim S.E., Esain V., Kwan W., Theodore L.N., Cortes M., Frost I.M., Liu S.Y, & North T.E. (2016). HIF1α-induced PDGFRβ signaling promotes developmental HSC production via IL-6 activation. Experimental hematology, 46, 83-95.e6.
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2

Imaging GFP Larvae with Lightsheet Microscopy

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lf:GFP larvae were anesthetized with 0.04 mg/ml tricaine methane sulfonate (MS-222) and imaged by fluorescence microscopy using a Zeiss Discovery V8/Axio Cam MRC. Lightsheet microscopy was performed using a Zeiss Lightsheet Z.1 at the Harvard Center for Biological Imaging (HCBI) core and analyzed using FIJI (NIH) as previously described59 (link).
Cox A.G., Hwang K.L., Brown K.K., Evason K., Beltz S., Tsomides A., O'Connor K., Galli G.G., Yimlamai D., Chhangawala S., Yuan M., Lien E.C., Wucherpfennig J., Nissim S., Minami A., Cohen D.E., Camargo F.D., Asara J.M., Houvras Y., Stainier D.Y, & Goessling W. (2016). Yap reprograms glutamine metabolism to increase nucleotide biosynthesis and enable liver growth. Nature cell biology, 18(8), 886-896.
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Imaging GFP Larvae with Lightsheet Microscopy

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lf:GFP larvae were anesthetized with 0.04 mg/ml tricaine methane sulfonate (MS-222) and imaged by fluorescence microscopy using a Zeiss Discovery V8/Axio Cam MRC. Lightsheet microscopy was performed using a Zeiss Lightsheet Z.1 at the Harvard Center for Biological Imaging (HCBI) core and analyzed using FIJI (NIH) as previously described59 (link).
Cox A.G., Hwang K.L., Brown K.K., Evason K., Beltz S., Tsomides A., O'Connor K., Galli G.G., Yimlamai D., Chhangawala S., Yuan M., Lien E.C., Wucherpfennig J., Nissim S., Minami A., Cohen D.E., Camargo F.D., Asara J.M., Houvras Y., Stainier D.Y, & Goessling W. (2016). Yap reprograms glutamine metabolism to increase nucleotide biosynthesis and enable liver growth. Nature cell biology, 18(8), 886-896.
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