Pac 300

Manufactured by Bio-Rad

Sourced in United States

The PAC 300 is a power supply unit designed for electrophoresis applications. It provides a steady and adjustable electric current to support various gel-based separation techniques. The device features multiple output channels and can be programmed to deliver specific voltage, current, and time settings to meet the requirements of different electrophoresis protocols.

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Lab products found in correlation

Hybond ecl extra, by Cytiva (1 mentions)

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PAC 300 power supply (3 citations)

3 protocols using pac 300

Analyzing Protein Expression via SDS-PAGE

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The electrophoretic experiments were performed by SDS-PAGE (10 % polyacrylamide unless specified otherwise) to analyze the YKL-40 and EMT related genes. Electrophoresis was conducted by a vertical gel electrophoresis device that was powered by (Mini PIII, Bio-Rad, Hercules, CA) a PAC 300 power supply (Bio-Rad). All SDS-PAGE samples (20 μg) were equilibrated in 10 mM Tris–HCl and 5 % SDS (pH 7.6) before loading.
Following complete separation, the gel was soaked briefly in a transfer buffer, which contained 25 mM Tris, 192 mM glycine, 20 % methanol, and 0.0375 % SDS (pH 8.3), for 30 s. The gel was then immediately electrotransfered to a nitrocellulose membrane (Hybond-ECL extra; Amersham) at 90 mA for 60 mins in a semi-dry Transfercell (Bio-Rad). The membrane was immersed in 2 % skim milk for 1 h with gentle agitation. After three washes with PBS for 5 mins each, the membrane was subjected to react with monoclonal or polyclonal antibodies and developed with chemiluminescence agents.

Jefri M., Huang Y.N., Huang W.C., Tai C.S, & Chen W.L. (2015). YKL-40 regulated epithelial-mesenchymal transition and migration/invasion enhancement in non-small cell lung cancer. BMC Cancer, 15, 590.

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Western Blot Analysis of LC3 and ERK

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After drug treatment, cells were washed twice with ice-cold PBS, and lysates from HGC-27 cells were prepared with 200 μl of NP-40 lysis buffer (2 mM Tris–Cl pH 7.5, 150 mM NaCl, 10% glycerol and 0.2% NP-40 plus a protease inhibitor cocktail (Li et al, 2014). The proteins were evaluated by using BCA assays and subjected to 13% SDS-PAGE by electrophoresis apparatus (Bio-Rad PAC300; Richmond, CA, USA), and electrotrasferred to nitrocellulose membrane (Beijing Biodee Biotechnology Corporation Ltd, Beijing, China). Membranes were blocked with 5% non-fat milk for 1 h at room temperature, and incubated overnight at 4 °C with primary antibodies (anti-LC3-I, anti-LC3-II, anti-ERK, anti-p-ERK primary antibody (1:100, 1:50, 1:100, 1:100, 1:50, 1:100). The bound primary antibody was detected by using appropriated HRP (horse radish peroxidase)-labeled secondary anti-rabbit or mouse IgG. Antibody bounds were detected by enhanced chemiluminescence.

Zhou S., Zhang R., Bi T., Lu Y, & Jiang L. (2016). INHIBITORY EFFECT OF LYCOPENE AGAINST THE GROWTH OF HUMAN GASTRIC CANCER CELLS. African Journal of Traditional, Complementary, and Alternative Medicines, 13(4), 184-190.

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BOX-PCR Fingerprinting of Bacterial Isolates

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Genomic DNA for BOX-PCR was extracted from all the isolates using a Bacterial DNA Extraction Kit (Sigma) following the instructions of the manufacturer and BOX-PCR fingerprint profiles generated using modifications of the methods described by Versalovic et al. (1994) and Trujillo et al. (2010) (link). To this end, Bioline 2x MiFi TM mix was used for PCR amplification in a final volume of 25 μl per reaction following the manufacturer's recommendations; the thermal cycling parameters were: 7 minutes at 95 °C, 30 cycles of 1 minute at 94 °C, 1 minute at 52 °C and 3 minutes at 72 °C followed by a final extension at 72 °C for 10 minutes. Five microliters of each PCR product was loaded onto a 2% agarose gel containing 10 μl of GelRed TM (Crisafuli et al. 2015) (link) per 100 ml and electrophoresis run at 70 V for 3 hours in freshly prepared 1x TBE-EDTA buffer at pH 8.0 using a Bio-Rad Pac 300 power supply; a DNA molecular weight marker 1kb HyperLadder TM (Bioline) was used as the molecular size standard. After electrophoresis, gels were photographed, stored on disk as TIFF files, and manually aligned into 9 multi-and 10 single-membered similarity groups.

Carro L., Razmilic V., Nouioui I., Richardson L., Pan C., Golinska P., Asenjo J.A., Bull A.T., Klenk H.P, & Goodfellow M. (2018). Hunting for cultivable Micromonospora strains in soils of the Atacama Desert. Antonie van Leeuwenhoek, 111(8).

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