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Mir 124 inhibitor

Manufactured by RiboBio
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Sourced in China

The MiR-124 inhibitor is a laboratory reagent designed to inhibit the expression of the miR-124 microRNA. MicroRNAs are small non-coding RNA molecules that play a crucial role in gene regulation. The MiR-124 inhibitor can be used in research studies to investigate the functions and effects of the miR-124 microRNA in biological systems.

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Lab products found in correlation

Mir 124 mimic, by RiboBio (5 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Interferin, by Polyplus Transfection (1 mentions) Lipo3000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Sh tgf β1, by RiboBio (1 mentions) Oe nc, by RiboBio (1 mentions) Sh nc, by RiboBio (1 mentions) Prestained protein marker, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000 lipo 2000 reagent, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 donkey anti mouse igg h l antibody, by Thermo Fisher Scientific (1 mentions) Polyvinylidene difluoride pvdf membrane, by Merck Group (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Inhibitor nc, by RiboBio (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay, by Promega (1 mentions) Mir nc, by RiboBio (1 mentions)

5 protocols using mir 124 inhibitor

1

Transfection of miR-124 and p38 siRNA

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MiR 124 mimic (Ribobio, Guangzhou, China), miR 124 inhibitor (Ribobio), p38 siRNA (1μg/μl, Ribobio) or their corresponding negative control (NC, Ribobio) were transfected into RAW264.7 cells with lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
Liang X., Wang L., Wang M., Liu Z., Liu X., Zhang B., Liu E, & Li G. (2020). MicroRNA-124 inhibits macrophage cell apoptosis via targeting p38/MAPK signaling pathway in atherosclerosis development. Aging (Albany NY), 12(13), 13005-13022.
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2

Manipulating circRNA_100782 and miR-124 in BxPC3 Cells

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INTERFERin (Polyplus transfection, Illkirch, France) was used for the transfection experiments with miRNA mimics or siRNAs according to the manufacturer’s instructions. miR-124 mimic, miR-124 inhibitor, negative control miRNA and siRNAs targeting circRNA_100782, STAT3 and siRNA negative control were obtained from RiboBio (Guangzhou, China). The knockdown efficiency of circRNA_100782 was evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Western blot was used to measure the efficiency of si-STAT3. To identify the role of IL6 in si-circRNA_100782 and miR-124-induced cell suppression, BxPC3 cells were treated with recombinant human IL6 cytokine (PeproTech, Rocky Hill, NJ, USA) after transfection with si-circRNA_100782 and miR-124 according to the manufacturer’s instructions.
Chen G., Shi Y., Zhang Y, & Sun J. (2017). CircRNA_100782 regulates pancreatic carcinoma proliferation through the IL6-STAT3 pathway. OncoTargets and therapy, 10, 5783-5794.
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3

miR-124 Regulates TGFβ1 and DNMT1 in Cells

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The cells in the logarithmic phase of growth were detached with trypsin and seeded in a six-well plate, at a density of 1 × 105 cells per well. After 24 h of conventional culture, the cells were transfected with 250 μl of Lipo3000 transfection reagent (Invitrogen) at room temperature for 0 to 15 min, and the solution was changed after 16 h. The RNA content was extracted after 48 h, followed by protein extraction after 72 h. The details of experimental grouping were described in the Results. mimic-negative control (NC), miR-124 mimic, inhibitor NC, miR-124 inhibitor, sh-NC, sh-TGFβ1, oe-NC, oe-TGFβ1, sh-DNMT1, and oe-DNMT1 were all purchased from Ribobio (Guangzhou, China).
Wang Y., Wang X., Zhang H., Han B., Ye Y., Zhang M., Wang Y., Xue J, & Wang C. (2021). Transforming Growth Factor-β1 Promotes M1 Alveolar Macrophage Polarization in Acute Lung Injury by Up-Regulating DNMT1 to Mediate the microRNA-124/PELI1/IRF5 Axis. Frontiers in Cellular and Infection Microbiology, 11, 693981.
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4

Evaluating miR-124 and CD151 Expression

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RPMI-1640, Dulbecco's Modified Eagle Medium (DMEM), and fetal bovine serum (FBS) were obtained from GIBCO (Grand Island, NY). Lipofectamine 2000 (Lipo 2000) reagent was obtained from Invitrogen (Life Technologies Corporation, Carlsbad, CA). The primers for miR-124 and U6 real-time PCR, miR-124 mimics, miR-124 inhibitor, CD151 siRNA, and their controls were purchased from RiboBio (Guangzhou, China). Antibody against CD151 (Cat No: ab33315) was procured from Abcam (Cambridge, MA). Antibody against Phospho-NOS3-S1177 (Cat No: AP0421) was purchased from ABclonal Biotech (Cambridge, MA). Prestained protein markers were obtained from Fermentas (Thermo Fisher Scientific Inc., Rockford, IL). Polyvinylidene difluoride (PVDF) membranes were from Millipore (Merck KGaA, Darmstadt, Germany). Horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence reagents were from Pierce Biotechnology (Thermo Fisher Scientific Inc., Rockford, IL). Alexa Fluor® 594 Donkey Anti-Mouse IgG (H+L) Antibody (Cat No: A-21203) were obtained from MOLECULAR PROBES (Thermo Fisher Scientific Inc., Rockford, IL). Other reagents were purchased from the Sigma-Aldrich Company, unless otherwise specified.
Zhao Y., Yan M., Chen C., Gong W., Yin Z., Li H., Fan J., Zhang X.A., Wang D.W, & Zuo H. (2018). MiR-124 aggravates failing hearts by suppressing CD151-facilitated angiogenesis in heart. Oncotarget, 9(18), 14382-14396.
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5

Luciferase activity analysis in 293-T cells

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For luciferase activity analysis, human 293-T cells were cultured in 24-well plates and transfected with 0.5 μg of either wide-type or mutant psiCHECK™2 Vector containing both firefly luciferase and renilla luciferase, together with miR-NC, miR-124 mimic, inhibitor-NC and miR-124 inhibitor (RiboBio, Guangzhou, China). Transfection was performed using Lipofectamine 3000 reagent (Invitrogen Life Technologies). The cell lysates were analyzed for luciferase activity 20 h post-transfection by the Dual Luciferase Reporter Assay (Promega) according to the manufacturer's instructions.
Zhao J., Pan Y., Li X., Zhang X., Xue Y., Wang T., Zhao S, & Hou Y. (2017). Dihydroartemisinin and Curcumin Synergistically Induce Apoptosis in SKOV3 Cells Via Upregulation of MiR-124 Targeting Midkine. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 43(2).
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