FFPE blocks were sectioned at a thickness of 4μm and a positive control was added on the right side of the slides. All immunostainings were fully calibrated on a Benchmark XT staining module (Ventana Medical Systems Inc., USA). Briefly, after sections were dewaxed and rehydrated, a CC1 Standard Benchmark XT pretreatment for antigen retrieval (Ventana Medical Systems) was selected for all immunostainings: Chromogranin A (1:500, Dako, Denmark), Synaptophysin, (1:200, Life Technologies, Invitrogen, USA), GAST, CGC and STT. Detection was performed with iView DAB Detection Kit (Ventana Medical Systems Inc., USA) and counterstained with hematoxylin (Ventana Medical Systems Inc., USA). After the run on the automated stainer was completed, slides were dehydrated in ethanol solutions (70%, 96%, and 100%) for one min each. Sections were then cleared in xylene for 2 min, mounted with Entellan and cover slips were added.
Cc1 standard benchmark xt pretreatment for antigen retrieval
Manufactured by Roche
Sourced in Denmark
The CC1 Standard Benchmark XT pretreatment for antigen retrieval is a laboratory equipment product. It is designed to facilitate the process of antigen retrieval, which is a crucial step in immunohistochemistry and other biological assays. The core function of this product is to prepare samples for subsequent analysis and detection of target proteins or antigens.
Automatically generated - may contain errors
Lab products found in correlation
Hematoxylin, by Roche (3 mentions)
Iview dab detection kit, by Roche (3 mentions)
Benchmark xt staining module, by Roche (3 mentions)
Synaptophysin, by Thermo Fisher Scientific (2 mentions)
Chromogranin a, by Agilent Technologies (2 mentions)
Brachyury, by Santa Cruz Biotechnology (1 mentions)
E cadherin, by Thermo Fisher Scientific (1 mentions)
Ultraview dab detection kit, by Roche (1 mentions)
E cadherin, by Roche (1 mentions)
β catenin, by Cell Marque (1 mentions)
β catenin, by Roche (1 mentions)
3 protocols using cc1 standard benchmark xt pretreatment for antigen retrieval
1
Immunohistochemical Staining of FFPE Samples
2
Immunohistochemical Staining Protocol for FFPE Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FFPE blocks were sectioned at 4 μm and a positive control was added to each slide. All IHC staining was fully calibrated on a Benchmark XT staining module (Ventana Medical Systems Inc., USA). Briefly, after sections were dewaxed and rehydrated, a CC1 Standard Benchmark XT pretreatment for antigen retrieval (Ventana Medical Systems) was selected for all immunostains: AMACR (P504S, ready to use, Biocare Medical, USA), β-catenin, (1:50, Cell Marque, USA), E-cadherin, (1:25, Life Technologies, Invitrogen, USA) and brachyury (1:200, Santa Cruz Biotechnology Inc., USA). Detection of AMACR, β-catenin and E-cadherin was performed with iView DAB Detection Kit (Ventana Medical Systems Inc., USA) and of brachyury with ultraView DAB Detection Kit (Ventana Medical Systems Inc., USA). Slides were counterstained with hematoxylin (Ventana Medical Systems Inc., USA) on an automated stainer and dehydrated in ethanol solutions (70%, 96%, and 100%) for one minute each. Before cover-slipping, sections were cleared in xylene for 2 minutes and mounted with Entellan (Surgipath, Germany).
3
Immunohistochemical Staining of FFPE Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FFPE blocks were sectioned at a thickness of 4μm and a positive control was added on the right side of the slides. All immunostainings were fully calibrated on a Benchmark XT staining module (Ventana Medical Systems Inc., USA). Briefly, after sections were dewaxed and rehydrated, a CC1 Standard Benchmark XT pretreatment for antigen retrieval (Ventana Medical Systems) was selected for all immunostainings: Chromogranin A (1:500, Dako, Denmark), Synaptophysin, (1:200, Life Technologies, Invitrogen, USA), GAST, CGC and STT. Detection was performed with iView DAB Detection Kit (Ventana Medical Systems Inc., USA) and counterstained with hematoxylin (Ventana Medical Systems Inc., USA). After the run on the automated stainer was completed, slides were dehydrated in ethanol solutions (70%, 96%, and 100%) for one min each. Sections were then cleared in xylene for 2 min, mounted with Entellan and cover slips were added.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!