An anti‐GFP antibody (#598) was purchased from MBL, Nagoya, Japan. An anti‐FLAG M2 antibody conjugated with peroxidase was purchased from Sigma (St. Louis, MO, USA). The anti‐β‐actin antibody (N21) and horse radish peroxidase (HRP)‐conjugated anti‐goat IgG antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti‐Akt (#9272), anti‐phospho‐Akt (Ser473) (#9271), anti‐CDCP1 (#4115), anti‐phospho‐CDCP1 (Tyr734) (#9050), anti‐ERK1/2 (#9102), anti‐phospho‐ERK1/2 (Thr202/Tyr204) (#9101), anti‐PKCδ (#2058), and anti‐phospho‐PKCδ (Tyr311) (#2055) antibodies were purchased from Cell Signaling Technology (Denvers, MA, USA). anti‐CDCP1 antibody for immunoprecipitation was originally developed as described previously.2
Anti β actin antibody n21
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Anti-β-actin antibody (N21) is a primary antibody produced by Santa Cruz Biotechnology. It is designed to detect the β-actin protein, which is a widely expressed cytoskeletal protein found in eukaryotic cells. This antibody can be used in various immunoassays, such as Western blotting, to identify and quantify the presence of β-actin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti cdcp1, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions)
Anti erk1 2, by Cell Signaling Technology (1 mentions)
Anti phospho akt ser473, by Cell Signaling Technology (1 mentions)
Anti phospho pkcδ tyr311, by Cell Signaling Technology (1 mentions)
Anti pkcδ, by Cell Signaling Technology (1 mentions)
Bradford protein assay, by Bio-Rad (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
2 protocols using anti β actin antibody n21
1
Antibody Procurement and Characterization
2
Immunoblotting Procedure for Protein Analysis
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Immunoblotting was performed according to the previous studies [15 (link), 16 (link)]. The immunoblotting was performed with whole cell lysates generated by RIPA buffer (Sigma Aldrich). Bradford protein assay (BioRad, Hercules, CA, USA) were used to determine the protein concentration of samples. 5 μg proteins per lane were loaded and separated on 10% SDS-polyacrylamide gel. The gel was transferred to a polyvinylidene difluoride (PVDF) membrane and probed with specific primary antibodies. Signals were detected after incubation with peroxidase-conjugated secondary antibody. Anti PARP-1 (46D11), cleaved PARP-1 (D214) and cleaved/active caspase3 (D175) primary antibodies were purchased from Cell Signaling. Anti β-Actin antibody (N-21) and secondary antibodies were bought from Santa Cruz. Protein bands were visualized using ECL reagents (Amersham, little Chalfont, UK) and exposed to X-ray film. Before probing with primary antibodies, the membranes were cut to avoid stripping.
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