Nano hplc easy nlc 1000

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Nano HPLC Easy-nLC 1000 is a high-performance liquid chromatography (HPLC) system designed for nano-scale applications. It features a compact design and delivers precise flow control for handling low-volume samples.

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Lab products found in correlation

Q exactive hesi orbitrap, by Thermo Fisher Scientific (2 mentions) Pepmap rslc c18, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

EASY-nLC 1000 (591 citations) Easy nLC (237 citations) EASY-nLC 1000 HPLC (21 citations) EASY‐nLC 1000 system (Nano HPLC (4 citations)

3 protocols using nano hplc easy nlc 1000

Proteomic Analysis of Trypanosoma cruzi EVs

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Purified EVs (20 μg) were diluted in 50 mM ammonium carbonate. Mass spectrometry analysis was carried out at Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Argentina, in a Q Exactive HESI-Orbitrap coupled to a nano HPLC Easy-nLC 1000 (Thermo Scientific). MS/MS data were used to search the all the available Trypanosoma cruzi databases at Tritrypdb (version 30) [11 (link)].

Caeiro L.D., Alba-Soto C.D., Rizzi M., Solana M.E., Rodriguez G., Chidichimo A.M., Rodriguez M.E., Sánchez D.O., Levy G.V, & Tekiel V. (2018). The protein family TcTASV-C is a novel Trypanosoma cruzi virulence factor secreted in extracellular vesicles by trypomastigotes and highly expressed in bloodstream forms. PLoS Neglected Tropical Diseases, 12(5), e0006475.

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Protein Extraction and HPLC Analysis

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One µg of each protein extraction was injected into a nano-HPLC Easy-nLC 1000 (Thermo Fisher Scientific, Massachusetts, United States). First, samples were concentrated using a precolumn PEPMAP100 C18 NanoViper Trap (Thermo Fisher Scientific, Massachusetts, United States). Then, samples were separated through a 50 cm column PEPMAP RSLC C18 (Thermo Fisher Scientific, Massachusetts, United States) on a gradient of ACN 5% to 40% (v/v) and formic acid 0.1% (v/v) for 120 min.

Poza-Viejo L., Redondo-Nieto M., Matías J., Granado-Rodríguez S., Maestro-Gaitán I., Cruz V., Olmos E., Bolaños L, & Reguera M. (2023). Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions. Scientific Reports, 13, 4951.

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Proteomic Analysis of T. cruzi

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After 10% SDS-PAGE of epimastigotes protein extracts, the section of the gel between 70 kDa and 100 kDa was manually cut and preserved in an eppendorf tube at -80°C until delivery for mass spectrometry analysis. Mass spectrometry analysis was carried out at Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Argentina, in a Q Exactive HESI-Orbitrap coupled to a nano HPLC Easy-nLC 1000 (Thermo Scientific). Resulting reads were matched to the T. cruzi proteome available at the UNIPROT proteome database.

Sternlieb T., Schoijet A.C., Genta P.D., Vilchez Larrea S.C, & Alonso G.D. (2021). An AMP-activated protein kinase complex with two distinctive alpha subunits is involved in nutritional stress responses in Trypanosoma cruzi. PLoS Neglected Tropical Diseases, 15(5), e0009435.

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