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Goat anti sm22α

Manufactured by Abcam
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Goat anti-SM22α is a primary antibody that recognizes the smooth muscle protein SM22α. It is commonly used in research applications for the detection and analysis of SM22α expression.

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Lab products found in correlation

Mouse anti α sma, by Merck Group (1 mentions) Bis tris gradient gel, by Thermo Fisher Scientific (1 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Enhanced chemiluminescence substrate, by Thermo Fisher Scientific (1 mentions) Rabbit anti mmp 9, by Abcam (1 mentions) Imaging system, by Bio-Rad (1 mentions) Rabbit anti opn, by Abcam (1 mentions) Sodium dodecyl sulfate polyacrylamide gel electrophoresis, by Ipsen (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Huabio (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) Methyl green, by Merck Group (1 mentions) Rabbit anti phosphorylated erk, by Cell Signaling Technology (1 mentions) Biotin conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Goat anti runx2, by R&D Systems (1 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Goat anti osteopontin, by R&D Systems (1 mentions)

3 protocols using goat anti sm22α

1

Immunoblotting for Cell Signaling

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Protein samples were separated by SDS-PAGE on 4–12% Bis-Tris gradient gels (Life Technologies), transferred onto nitrocellulose membranes (Bio-Rad), and incubated with primary antibodies, diluted 1:1,000 (unless noted): rabbit anti-HMGA1 (1:10,000), rabbit anti-vimentin (1:4,000), mouse anti-phospho vimentin (1:250), mouse anti-PECAM-1, rabbit anti-Snail/Slug, rabbit VE-cadherin, goat anti-SM22α (Abcam), mouse anti-αSMA (Sigma-Aldrich, St Louis, MO), mouse anti-GAPDH, mouse anti-β-actin (Santa Cruz Biotechnology, Dallas, TX), and mouse anti-BMPR2 (1:200, BD Biosciences, Franklin Lakes, NJ).
Hopper R.K., Moonen J.R., Diebold I., Cao A., Rhodes C.J., Tojais N.F., Hennigs J.K., Gu M., Wang L, & Rabinovitch M. (2016). In Pulmonary Arterial Hypertension, Reduced BMPR2 Promotes Endothelial-to-Mesenchymal Transition via HMGA1 and its Target Slug. Circulation, 133(18), 1783-1794.
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2

Western Blot Analysis of Rat Aortic SMCs

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Proteins were removed from rat aortic SMCs following lysis and after intervention. The cells were divided into four groups, as described, and 40 µg of protein per lane was separated using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Epizyme, Beijing, China), transferred to a polyvinylidene difluoride membrane (Millipore Sigma, Billerica, MA, USA), and blocked with western quick-blocking buffer (Beyotime) for 15 min at room temperature. The blocked membranes were then incubated overnight with primary antibodies, namely goat anti-SM22α (1:500; Abcam, Cambridge, UK), rabbit anti-OPN (1:1000; Abcam), and rabbit anti-MMP9 (1:1000; Abcam). After 12 h to 14 h, the membranes were incubated with a horseradish-peroxidase-conjugated secondary antibody (HuaBio, Hangzhou, China) for 1 h at room temperature. The immunoblots were probed using an enhanced chemiluminescence substrate (Thermo Fisher Scientific), and an imaging system (Bio-Rad Laboratories, Hercules, CA, USA) was used for blot detection and recoding of chemiluminescence. The results were normalized to that of GAPDH, and experiments were performed in triplicate.
Liu Y., Liu P., Song Y., Li S., Shi Y., Quan K., Yu G., Li P., An Q, & Zhu W. (2021). A heparin–rosuvastatin-loaded P(LLA-CL) nanofiber-covered stent inhibits inflammatory smooth-muscle cell viability to reduce in-stent stenosis and thrombosis. Journal of Nanobiotechnology, 19, 123.
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3

Vascular Calcification Analysis in Mice

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Innominate arteries from 12 week old ApoE−/−OPG−/− and ApoE−/−OPG+/+ mice were fixed in formalin and embedded in paraffin. Five micron thick sections were used for histochemical and immunohistochemical analyses. Alizarin Red S or Von Kossa staining was used to detect calcium deposition. The following antibodies were employed: anti goat SMα-actin (Sigma, St Louis, MO), goat anti SM22α (Abcam, Cambridge, MA), rabbit anti phosphorylated ERK (CellSignaling, Danvers, MA), goat anti Runx2 (R&D Systems, Minneapolis, MN), mouse anti RANKL (Imgenex, San Diego, CA), goat anti Osteopontin (R&D Systems, Minneapolis, MN), rabbit anti cleaved caspase-3 (CellSignaling, Danvers, MA), control rabbit, goat and mouse IgG (Invitrogen, Carlsbad, CA), biotin conjugated rabbit anti-goat IgG (Pierce Thermoscientific, Rockford, IL), biotin conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA), biotin conjugated rabbit anti-mouse IgG (Invitrogen, Carlsbad, CA). Sections were counterstained with haematoxylin (Ricca Chemical Company, Arlington, TX) or Methyl Green (Sigma).
Callegari A., Coons M.L., Ricks J.L., Rosenfeld M.E, & Scatena M. (2014). Increased Calcification in Osteoprotegerin Deficient Smooth Muscle Cells: Dependence on Receptor Activator of NF-κB Ligand and Interleukin-6. Journal of vascular research, 51(2), 118-131.
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